The CELTN showed higher level cellulytic activity of 12464 Umg-1 as compared to 1536 and 1219 Umg-1 for CelB and CelA from Thermotoga neapolitana (Bok et al., 1998), 735 Umg-1 for GH12 endoglucanase from Thermotoga petrophila (Haq et al., 2015), 66 Umg-1 for thermostable Cellobiohydrolase
from Thermotoga petrophila (Haq et al., 2018) and 4.23 Umg-1 for hyper-thermostable endoglucanase from Thermotoga meritima (Zhang et al., 2015).
Silva, "Evidence of cAMP involvement in cellobiohydrolase
expression and secretion by Trichoderma reesei in presence of the inducer sophorose," BMC Microbiology, vol.
Keyword: Glycoside hydrolase family 6; Cellobiohydrolase
; Heterologous expression; Thermostable enzyme; Bioconversion
hydrolyzes crystalline cellulose on hydrophobic faces," The Journal of Biological Chemistry, vol.
. -- Cellobiohydrolase
, EC 22.214.171.124) activity was measured by combining in separate test tubes 2.0 mL of enzyme with 20 mg of microcrystalline cellulose (type 50, Sigma Chemical Co.) in 1.0 mL of 0.05 M sodium citrate buffer (pH 5.0) and incubating the reaction mixture for 120 minutes at 40[degrees]C.
Wood biofilms were assayed for the activity of cellobiohydrolase
(CBH, EC [Enzyme Commission number] 126.96.36.199), an extracellular enzyme involved in the degradation of lignocellulose, as outlined by Sinsabaugh et al.
There are three major types of enzymes in cellulase system: [[beta]-1,4-endoglucanase (endocellulases, EC 188.8.131.52), which randomly hydrolyze internal bonds), cellobiohydrolase
(exocellulases, EC 184.108.40.206), cleave the ends of the cellulose chains results in cellotetraose or cellobiose) and [beta]-glucosidases (EC 220.127.116.11), which produce monosaccharide, glucose from cellobiose .
The same situation was found in the strain Cellvibrio mixtus that possesses 9 endoglucanases and 12 [beta]-glucosidases but no cellobiohydrolase
. Because of the lack, strain PR1 and Cellvibrio mixtus could not grow on cellulose.
Three-dimensional structure of cellobiohydrolase
II from Trichoderma reesei.
Shu, "Production and distribution of endoglucanase, cellobiohydrolase
, and [beta]-glucosidase components of the cellulolytic system of Volvariella volvacea, the edible strawmushroom," Appliedand-Environmental Microbiology, vol.
Trametes versicolor produces a multicomponent cellulase system, consisting of endoglucanase (EC 18.104.22.168), cellobiohydrolase
(EC 22.214.171.124), and [beta]-glucosidase (EC 126.96.36.199) (Cai et al.
, endoglucanase and xylanase activities were detected in fluids collected from cultures containing sodium carboxymethyl cellulose (CMC) or xylan as carbon sources and enzyme inducers.