Results demonstrated differences between the performed tests by VITEK[R]2 and conventional tests with regard to cellobiose
Huang, "Conversion of cellulose and cellobiose
into sorbitol catalyzed by ruthenium supported on a polyoxometalate/metal-organic framework hybrid," ChemSusChem, vol.
In addition, the stp1 exhibited a higher level of expression in sophorose than in cellulose, although it is involved in cellobiose
and glucose transport .
Then, 2.5 mL of the culture ([OD.sub.600] = 1.9) was transferred to YPGGC (3.0 g [L.sup.-1] yeast extract, 5.0 g [L.sup.-1] peptone, 20.0 g [L.sup.-1] galactose, 20.0 g [L.sup.-1] glucose and 10.0 g [L.sup.-1] cellobiose
) and cultured under the same conditions.
In the first screening, twenty-three yeast strains were assessed individually for their ability to grow in medium containing CMC, Avicel[R], cellobiose
and xylan from Birchwood (Sigma) as sole carbon sources (Table 1).
all amino acids and a number of sugars, such as trehalose, cellobiose
, maltose, sucrose, glucose, and fructose, were tested.
stipitis does not require the addition of vitamins to the fermentation of xylose and it is able to use different sugars as substrates, such as glucose and cellobiose
(Agbogbo & Wenger, 2007; Bellido et al., 2011).
Puri, "Immobilization of [beta]-glucosidase on a magnetic nanoparticle improves thermostability: application in cellobiose
hydrolysis," Bioresource Technology, vol.
Matsumura et al., "A novel combined thermometric and amperometric biosensor for lactose determination based on immobilised cellobiose
dehydrogenase," Biosensors and Bioelectronics, vol.
where C was the concentration of hydrolysis product, Ss was the peak area of standard sugar sample, S was the peak area of hydrolysis product, and [C.sub.s] was the concentration of standard sugar sample, including glucose, xylose, and cellobiose
The pretreatment that had the greater effect for the transformation of cellulosic material to the accessible enzyme was saccharification of dilute acid, a pretreatment that generates sugars such as xylose (0.07069 g xylose/g biomass) and cellobiose
(0.02946 g cellobiose/g biomass).
It was positive for glucose, sorbitol, rhamnose, L-arabinose, melibiose, lysine, ornithine, citrate, galactoside, and cellobiose
tests and negative for sucrose, raffinose, inositol, adonitol, urea, hydrogen sulfide, indole, arginine, tryptophan deaminase, aesculin, acetoin, malonate, tartrate, acetamide, cetrimide, and Voges-Proskauer tests.