9] reported the purification of a novel cellobiose
of Cellulomonas biozotea with a molecular mass of 109 kDa as determined by SDS-PAGE.
This bacteria was found to be able to hydrolyze various carbohydrates, including L-arabinose, galactose, fructose, mannose, [alpha]-methyl-D-glucoside, N-acethyl-glucosamine, D-turanose, salicin, cellobiose
, [beta]-gentiobiose, and D-xylose (Table 1), but did not utilize D-arabinose, erythritol, sorbose, dulcitol, inositol, [alpha]-methyl-D-mannoside, Lactose, D, L-arabitol, 2-keto-gluconate, or 5-keto-gluconate (Table 1).
The CMCase activities determined in our study were comparable to those reported for the anaerobic rumen fungus Neocallimastix frontalis EB188, which was originally incubated in glucose medium and switched to glucose, cellobiose
, or cellulose medium (Barchievich and Calza, 1990).
8 [micro]m Growth Glucose + + Xylose + + Arabinose + + Cellobiose
+ + Avicel - - Enzyme activities CMCase - + Xylanase + + Arabinofuranosidase + +
2 g of glucose and cellobiose
were added to 100 ml medium to supply sufficient carbohydrate source.
Tenders are invited for Supply of Item Potassium phosphate dibasic 500G, Dried yeast extract 500G, Tween 80 500ml, Peptone powder 500G, Trichloro acetic acid 100G, midoblack 25G, Acetic acid 500M,L Mueller hinton agar 500G, Dextrose 50 discs per vial Vial, Maltose 50 discs per vial Vial, Sucrose 50 discs per vial Vial, Lactose 50 discs per vial Vial, Galactose 50 discs per vial Vial, Melibiose ,50 discs per vial Vial, Cellobiose
50 discs per vial Vial, Inositol 50 discs per vial Vial, Xylose50 discs per vial Vial, Raffinose 50 discs per vial Vial etc.
API 50 CH fermentation patterns of isolated LAB from the control WCWS Items Group A Group B Group C ZZU 3 ZZU 21 ZZU 16 ZZU 8 L-Arabinose + + + + Ribose + w - + D-Xylose + + - + Galactose + + - - D-Glucose + + + + D-Fructose + + + + D-Mannose + + + + Mannitol - - - - Sorbitol - - - - [alpha]-Methyl-D-manno side - - - - [alpha]-Methyl-D-glucoside + + w - V-acetyl glucosamine + w + w Amygdaline - - + + Arbutine - w w w Esculine - - - - Salicine + w + w Cellobiose
+ + + + Maltose + + + + Lactose - w - - Melibiose + + - - Saccharose + + w + Trehalose + + + - Melezitose - - - - D-Raffinose + + - - Starch w - - - [beta]-Gentiobiose w w w - D-Turanose + + + - D-Arabitol - - - - Gluconate - - w w 5-ceto-gluconate - - w - Items Group D Group E Group F JCM ZZU 12 ZZU 23 ZZU 20 [5805.
Furthermore, phenotypic tests such as acid production from cellobiose
and citrate utilization, previously proposed to be useful for identification of E.
They use disaccharides including cellobiose
, maltose and lactose but not sugar alcohols.
Dibasic sodium phosphate, sodium carbonate, Folin-Ciocalteu reagent (FCR), sodium hydroxide, sodium tetraborate decahydrate, 1-butyl-3-methylimidazolium chloride, nitric acid, and hydrogen peroxide as well as standard compounds catechin, piceid (polydatin), transresveratrol, quercetin, myricetin, quercetin glucoside, kaempferol, 3, 4-dihydroxybenzoic acid (3, 4-DHB), caffeic acid, syringic acid, salicylic acid, ferulic acid, gallic acid, sinapinic acid, myo-inositol, arabitol, glucitol, mannitol, fucose, cellobiose
, galactose, glucose, fructose, arabinose, xylose, ribose, acetic acid, were purchased from Sigma-Aldrich (Germany or USA).
It did not produce acid from D- glucose, D-mannose, D-fructose, maltose, D-mannitol, rhamnose, sucrose, D-trehalose, D-galactose, cellobiose
, D- raffinose, xylose, lactose, ribose, arabinose, D-melezitose, D-salicin and aesculin.
Novel interaction between laccase and cellobiose
dehydrogenase during pigment synthesis in the white rot fungus Pycnoporus cinnabarinus.