Conclusions: The present findings revealed that peroxidase extracted from Citrus reticulata var Kinnow is very much stable at higher temperatures as well as against chaotropic agent
Given that during the refolding process by dialysis, as well as dilution of the chaotropic agent
the protein was also diluted, precipitation with trichloroacetic acid (TCA) was carried out before quantification.
(12,13) Traditional methods for measuring antibody affinity or avidity are cumbersome; however, inclusion of a chaotropic agent
to dissociate low-avidity antibodies in an EIA can provide avidity information in a simple assay.
After thawing and a 1-minute centrifugation at 500 x g, approximately 0.2 g of the tissue pellet was digested by mechanical disruption and the DNA was purified by adsorption to glass milk in the presence of a chaotropic agent
, according to the manufacturer's instructions (Fast Prep, Bio 101, Vista, CA).
Because previously reported chromatographic conditions (8,15,16) could not be applied directly to CSF analysis, we evaluated the optimum conditions by studying the effects of different salts, such as lithium, sodium, and potassium phosphate buffers; amines such as triethylamine (TEA), trimethylamine, morpholine and guanidine thiocyanate; and the chaotropic agent
potassium perchlorate in different concentrations in mobile phase A.