A mixture of 3 (2.39 g, 10 mmole), anhydrous [K.sub.2]C[O.sub.3] (1.38 g, 10 mmole), ethyl chloroacetate
(1.225 g, 10 mmole), and dry acetone (30 ml) was heated for 8 h (TLC) under reflux.
(f), (g), and (h) Staining with chloroacetate
esterase reaction was performed to detect neutrophil granulocytes.
On the other hand, when 1 was refluxed in dimethylformamide with ethyl chloroacetate
, the ester 2b was abstained and its structure was confirmed with different spectral data: the presence of the ester C=O at 1730 [cm.sup.-1] in IR, for example, and the presence of C[H.sub.2]C[H.sub.3] in H-NMR as quartet and triplet at [delta] 3.2,1.4 ppm, respectively.
In cryosections, naphthyl AS-D chloroacetate
and Tween-80 did not yield any colorimetric reactions at any of the three pHs, whereas [alpha]-naphthyl acetate yielded strongly positive results at pH 7.0 only (Fig.
Mast cells were detected by chloroacetate
esterase (CAE) staining according to published protocols of mast cell detection (Beckstead et al., 1981).
Many bacteria strains that possess inducible dehalogenases have ability to cleave off carbon-halogen bond in chloroacetate
were isolated from soil (Hardman and Slater, 1981; Ismail et al., 2008).
Aminabhavi, "Density, viscosity, refractive index, and speed of sound in the binary mixtures of ethyl chloroacetate
with aromatic liquids at 298.15, 303.15, and 308.15K," Journal of Chemical and Engineering Data, vol.
Then ethyl chloroacetate
(1.22 mL, 0.01 mole) was added to the reaction mixture and stirred for 6 h.
In our previous work we studied the kinetics of pH-independent hydrolysis of 4-nitrophenyl chloroacetate
(4-NPCA) under ultrasound by using a spectrophotometric method .
General procedure for synthesis of compound 1: A mixture of phenyl tetrazole 5 g (0.03 mol) in methanol is prepared, stirred well to dissolve compound 1.To this add 3.67 mL (0.03 mol) of ethyl chloroacetate
drop wise with continuous stirring to get clear solution.
The clinician should be alerted to the possibility of this diagnosis by a finding of a less differentiated neoplasm combined with pathologic evidence of myeloid differentiation in the presence of positive testing for chloroacetate
esterase, myeloperoxidase, lysozyme, CD43, CD79a, or CD3.