The rate of the conversion of CPRG to chlorophenol red
(CPR) was measured bichromatically at 577 and 700 nm.
Estrogenic activity from the enzymatic hydrolysis of chlorophenol red
[beta]-D-galactopyranoside was read at 540 nm using a colorimetric assay.
This [beta]-galactosidase activity is quantified at 540 nm by the conversion of the chromogenic substance chlorophenol red
-[beta]-D-galactopyranoside into chlorophenol red
Other features include auto shut-off after 10 minutes of non-use, self-diagnostic and error messages, chlorophenol red
method and is a technological alternative to chemical test kits.
[beta]-D-galactopyranoside (CPRG) and p-nitrophenylphosphate (PNPP) were purchased from Roche (Mannheim, Germany).
[beta]-D-galactopyranoside (CPRG) was obtained from Roche Diagnostics (Mannheim, Germany).
The supernatant containing the CsA-antibody-enzyme complex is then transferred to a measuring cuvette, where it is mixed with chlorophenol red
galactoside, which is hydrolyzed by the [beta]-galactosidase to chlorophenol red
CPRG is converted to chlorophenol red and reflects [Beta]-galactosidase activity, which is indicative of the estrogenic activity.
conversion of the chromogenic substance chlorophenol red-[Beta]-D-galactopyranoside (CPRG) into chlorophenol red by the action of the reporter enzyme [Beta]-galactosidase.
This [Beta]-galactosidase activity is quantified by the conversion of the chromogenic substrate CPRG into chlorophenol red.
Upon binding of the receptor protein to ERE, [Beta]-galactosidase is expressed and secreted into the culture medium where it reacts with its substrate chlorophenol red [Beta]-D-galactopyranoside (CPRG) to cause a color change from yellow to red (5).
Chlorophenol red [Beta]-Dgalactopyranoside (CPRG) was obtained from Boehringer (Mannheim, Germany).