are taken up by the pellicle and the color imparted is determined by the natural color of the chromogen
and the color seen on the tooth is considered to be derived mostly from polyphenolic compounds (15,16).
The principle of this method is based on staining of chromogen
DAB which will bind with the antibody of p53, Bcl-2, Caspases 8 and 9, and formed brown color on the cell membrane.
was applied for 20 minutes and all the slides were counter-stained with haematoxylin dehydrated and mounted.
The medium also possesses an enhanced dual chromogen
system that ensures colonies of Salmonella are always green while non-target organisms appear black or colourless, for clear differentiation.
After washing the wells, a chromogen
substrate (tetramethylbenzidine) is added and incubated, leading to color development which is proportional to the amount of malaria antibodies present in the sample.
In the presence of an acidic buffer with a pH of 5.2 (S2) and a suitable oxidant (Fe[Cl.sup.3+]) (S3), a stable and colored radical solution is formed by the chromogen
that contains 4-amino-N, N-diethylaniline sulfate.
DMC possesses carbonyl group (C=O) of the chromogen
(Ar-COCH=CH-Ar) as an intermediate which can produce deeper colour during the reaction by a phenomenon known as "Halochromy" .
The peroxidase reaction was developed using 3-amino-9-ethylcarbazol (AEC) as a chromogen
and the immunostained sections were screened under a light microscope.
3-Amino-9-ethylcarbazole (AEC, Vector Laboratories, Burlingame, CA, USA) was used as chromogen
and slides were slightly counterstained with Mayer's hematoxylin.
Creatinine with picric acid in alkaline environment (NaOH) creates the chromogen
absorbance was measured at wave length of 450 nm using Creatinine Analyzer 2 (Beckman, Munich, Germany).
CPCs were detected using a monoclonal antibody directed against PSA, clone 28A4 (Novocastra Laboratory, UK), and identified using an alkaline phosphatase-anti alkaline phosphatase-based system (LSAB2, DAKO, USA), with New Fuschin as the chromogen
. Positive samples underwent a second process with anti-P504S clone 13H4 (DAKO, USA) and identified with a peroxidase-based system (LSAB2, DAKO, USA) with DAB (3,3'diaminobenzidine tetrahydrochloride) as the chromogen
, according to the manufacturers' instructions.
reagent (DAB) was added to the tissue sections.