Biotin would bond with horseradish peroxide enzymes and DAB chromogen
and forms brown-colored complex at that bond.
The amount of sandwiched histidine proteins was proportional to the color intensity following the addition of a chromogen
substrate into the ELISA wells.
Diaminobenzidinetetrahydrochloride (Fluka Chemie GmbH, Buchs, Switzerland) was used as chromogen
, and slides were counterstained with Papanicolaou solution (Merck GmbH, Darmstadt, Germany).
In addition, Harlequin Listeria Chromogenic Agar uses a novel chromogen
to distinguish L.
This dual chromogen
medium enables the isolation of salmonella (including Salmonella typhi and S.
was added to the sections for 5 minutes to give color to the antigens and sections were again washed with TBS.
Briefly, 20 ul of serum sample (or standard, 6-hydroxy-2,5,7,8- tetramethylchroman-2-carboxylic acid) was incubated with 1 ml of chromogen
(consisting of metmyoglobin and ABTS(r)) at 37degC in a cuvette in temperature-controlled spectrophotometer.
In the Morgan-Elson reaction the NAG in the reducing end is successfully transformed into a chromogen
under alkaline condition at 100[degrees] C and subsequently by the action of p-dimethyl amino benzaldehyde (p-DMAB) in glacial acetic acid and hydrochloric acid mixture (9:1; v/v) to give a reddish purple colored product that can be detected at 585 nm.
Diaminobenzidine tetrahydrochloride was used as a chromogen
to localise the peroxidase.
Assessment of chromogen
suitability in ELISA for the detection of anaplasmosis and tripanosomosis.
TNF-[alpha] and NF-[kappa]B were visualized with the chromogen
3,3 diaminobenzidine (DAB) and counterstained with Harry's hematoxylin, mounted, and assessed by light microscopy.
Oxford, UK) was used for detection of marked cells and AEC as chromogen