As shown in Table 2, diabetes caused increases in the activities of glucose-o-phosphate dehydrogenase (17%) carnitine acetyltransferase (21%), 2-oxoglutarate dehydrogenase (37%), and fatty acid synthetase (62%), but we found no significant changes in citrate synthase
activity (Table 2).
In all fibroblast cultures from PDHc-deficient patients, citrate synthase
activity was within the range of values obtained for the controls and was used as the mitochondrial reference enzyme (data not shown).
Extracted mitochondrial pellets from healthy controls and from patients with a defect in complex III (all assayed for complex III, citrate synthase
, and protein) were kept under different conditions (duration and temperature) to determine the stability of the complex III enzyme and the repeatability of the assay.
Mitochondrial enzyme specific activities in spermatozoa from control and asthenozoospermic individuals Enzymatic Control subjects (a) Asthenozoospermic activities subjects (a) Citrate synthase
Comparison of partial citrate synthase
gene (gltA) sequences for phylogenetic analysis of Bartonella species.
gene sequence: a new tool for phylogenetic analysis and identification of Ehrlichia.
sibirica mongolitimonae citrate synthase
A gene (GenBank accession nos.
0 Real-Time PCR System (Roche Diagnostics GmbH) by using an LC Taqman Master Kit (Roche Diagnostics GmbH), primers, and TaqMan probe targeting the citrate synthase
Although cellular culture of both specimens and molecular testing of the blood sample were negative, PCR for the sequences of citrate synthase
(GenBank accession no.
Amplification and sequencing of 1,096-, 3,005-, 588-, and 4,918-bp products for the citrate synthase
(gltA), surface cell antigen (sca4), outer membrane protein A (ompA), and ompB genes, respectively, were conducted by using primers previously described (9).
primers targeted the RNA polymerase [beta]-subunit-encoding gene (rpoB) and the citrate synthase
gene (gltA) (3-5).
The diagnosis of murine typhus was established by PCR for Rickettsia 17 kDa and citrate synthase
(gltA) genes as described previously (6,7).