Unlike conventional cloning technology, the gateway technology provides an accurate way on the basis of bacteriophage-A to transfer DNA fragments between cloning vectors
and allows precise cloning without altering the coding sequence in both donor and destination vectors because of the presence of ccdB gene [24, 25].
The plasmid pUC19 is one of a series of plasmid cloning vectors
and it is one of the most widely used vector molecules as the cells into which foreign DNA has been introduced, can be easily distinguished from the non-recombinants based on color differences of colonies on growth media (Storkle and Dominic (2007).
A series of plasmid cloning vectors
were preceded by the letter "p".
With primers 8F and 1391R which are relevant to bacterial species, the PCR of 16S rDNA was amplified using cloning vectors
specifically that of PCR4.0, the species were then imported into a database and aligned against E.
PCR products can be ligated directly into pGEM-T Easy (Promega) or PCR 2.1-TOPO (Invitrogen) cloning vectors
This cloning method allows transferring of DNA fragment into different cloning vectors
without using restriction endonucleases and ligase.
used for transformation was pBI121 for overexpression construct, pGEMT-Easy vector for primary cloning of TYDC and Cor and pTZ57R/T vector for insertion genes of interest in E.
After moving to National Institutes of Health as Visiting Scientist in 1983, he developed a high-efficiency transfection method for delivering cDNA libraries into mammalian cells and cDNA cloning vectors
that enable selection on stable cDNA expression.
Most plasmid cloning vectors
carry at least one gene that confers antibiotic resistance on the host cell, and selection for transformants is achieved simply by plating on to an agar medium that contains ampicillin antibiotic.
A pSC101-derived plasmid which shows no sequence homology to other commonly used cloning vectors