Estimates of genetic parameters and genetic change for reproduction, weight, and wool characteristics of Columbia sheep
Samples were inoculated immediately into tryptic soy broth and incubated at 36[degrees]C for 24 hours; samples were subcultured onto 5% Columbia sheep
blood agar and incubated for 48 hours.
Before each experiment, two subcultures on Columbia sheep blood agar (bioMerieux, Craponne, France) were performed for 48 h at 36 [degrees]C, under anaerobic conditions (Anoxomat Mart system, Mart Microbiology B.V., Netherlands).
The samples were homogenized and serially diluted (10-fold dilutions), and 100 pt\ of each dilution were spread on Columbia sheep blood agar and incubated at 36 [degrees]C under anaerobic conditions for 5 days.
Stored isolates were subsequently thawed and sub-cultured onto Columbia sheep
blood agar (Fort Richard Laboratories, Auckland).
These tests were wet smear microscopy and incubation on 5% Columbia sheep
blood agar (Becton Dickinson, Heidelberg, Germany) in an atmosphere of 5% C[O.sub.2] at 35[degrees]C for 1 or 2 days.
The swabs were inoculated in Columbia sheep
blood agar and selective enrichment broth.
Animals can be registered with the Columbia Sheep
Breeders Association of America.
The standard breeds of these species (in my case Columbia sheep
) can also be raised relatively "pesticide free"!
Heart, liver, spleen, and lung samples were inoculated onto Columbia sheep
blood agar plates (Oxoid GmbH, Wesel, Germany) and Brolacin agar plates (Merck KGaA, Darmstadt, Germany) for the isolation of bacterial pathogens or Sabouraud 4% dextrose agar plates (Merck KGaA) for fungal culture.
After 4 days, a gram-variable polymorphic rod, named nimes373, grew from both aerobic culture vials and yielded very small polymorphic colonies on Columbia sheep
blood and blood-chocolate agar plates (Bio-Rad, Marnes-la-Coquette, France).
Necrotic tissues from both cases were cultured on Columbia sheep
blood agar plates incubated aerobically and anaerobically (ANOXOMAT system, MART Microbiology BV, Lictenvoorde, the Netherlands) at 37[degrees]C for 24 h and 48 h, respectively.