Intermediate cloning vector pEASY-T Simple, competent cell
TOP 10 and high fidelity Trans Taq-T DNA polymerase were purchased from Transgen Biotech Company.
2]) solution to yield the final competent cell
During the heat shock period the motion of tiny plasmid DNA molecules in the competent cell
mixture is likely to increase.
Then, the competent cell
was transferred to the LB medium that contained kanamycin (antibiotic), X-gal and IPTG.
The DNA movement into competent cell
is stimulated by briefly raising the temperature to 42[degrees]C.
Almost any competent cell
biologist or geneticist can attempt to produce human iPS cells.
The United States District Court of the Western District of Texas (Austin Division) issued a summary judgment ruling on November 2, 2001 in favor of Stratagene (La Jolla, CA; 858-592-9900), holding that Stratagene's chemically competent cell
products do not infringe Invitrogen's U.
The yeast strain used in this study was the YRG-2 competent cell
line (MATa ura3-52 his3-200 ade2-101 lys2-801 trp1-901 leu2-3 112 gal4-542 gal80-538 lys2::[U A S.
The purified c-FLIP(L) genes were ligated with pMD19-T vector (Takara, Dalian, China) and then were tranformed into the competent cell
coli competent cells
with high transformation efficiency, however there is dearth of knowledge on simple, reliable and more efficient method to prepare high efficiency competent cell
The 5 ml of overnight competent cell
culture was then put in the conical flask containing 50 mL antibiotic free SOB medium supplemented with 10 mM MgSO4.