Increased number of chromatin bridges in anaphase has been described for condensin II knockout cells as well as condensins I and II depleted cells [39, 40].
In vertebrates, condensins I and II are both composed of the SMC2/4 heterodimer together with distinct additional non-SMC subunits, CAP-G/G2, CAP-D2/D3, and CAPH/H2 .
Bosco, "Condensins are required for maintenance of nuclear architecture," Cells, vol.
SMC4 which forms a complex with SMC2 in condensin was also pulled down by GST-Nesprin-2-SMC.
As condensin exists in two complexes, condensin I and condensin II , we used CAP-H (kleisin [gamma], non-SMC condensin I complex subunit H) and CAP-H2 (kleisin [beta], non-SMC condensin II complex subunit H2) antibodies to probe the GST-Nesprin-2-SMC pulldown and identified CAP-H and CAP-H2 in the precipitate (Figure 3(c)).
Nesprin-2 Knockdown Does Not Affect Condensin Distribution.
In summary, we report a novel interaction partner of Nesprin-2 giant and show that the Nesprin-2 condensin interaction has an impact on mitotic chromosomes.
Alternatively, the interaction between condensin and Nesprin-2 is an indirect one.
Condensins, proteins which play a major role in chromosome condensation and DSB repair during prophase I, are also known to play a role .
Amon, "Condensins promote coorientation of sister chromatids during meiosis I in budding yeast," Genetics, vol.
As the chromosomes condense, REC8 is cleaved on the chromosome arms and the cohesin complex is replaced by the condensin complex.