In order to study substitutions along NS5B protein, Uruguayan HCV AA sequences were aligned to the NS5B world consensus sequences
. Almost full-length AA sequences were obtained in 26 out of 31 analyzed strains.
(@) Number of hits: *, 1-10; **, 11-99; ***, 100-1000; ***, 1000-3500; Sp1 consensus sequence
5'-(G/T)GGGCGG(G/A)(G/A)(C/T)-3' is underlined; G-runs are highlighted by red color.
List of heat shock elements deduced, their position and matching percent accuracy to the consensus sequence
A Chr 1 consensus sequence
was constructed for each strain using the SNP information from MGP.
Consequently, there is a clear need for a NGS based HLA genotyping method and analytical pipeline that not only can generate accurate HLA genotyping information, but also generate an accurate, fully phased HLA consensus sequence
for both genotyped alleles.
Therefore, the consensus sequences
were generated and used in the further analysis.
In order to compare the consensus sequence
(~1400bp) with other sequences available on GenBank database <http://www.ncbi.nlm.nih.
with sequences obtained Samples Primer Access number T.pretiosum ITS-2- R DQ525178.1 T.
In the acidic lipase, there are four consensus sequences
for N-glycosylation of the enzyme which is Asn-X-Ser/Thr (Fig.
The sequencing platforms will allow the researchers to cross-validate consensus sequences
to produce the highest possible genome sequence accuracy.
pIL-6P-luc710 contains the four consensus sequences
for the transcription factors activating-protein-1 (AP-1), cAMP-responsive element binding protein (CREB), CCAAT-enhancer binding protein-[beta] (C/EBP-[beta]), and nuclear factor [kappa]-light-chain-enhancer of activated B cells (NF-[kappa]B) as pIL-6P-luc1184.