The IncX4 plasmid harboring mcr-1 (pEc36_mcr-1) was divided into 2 contigs
, which we subsequently cloned by using PCR and sequencing.
The singleton NEF-17-1a-T3_K07 and contig
1 were matched to mannanase A from Piromyces sp.
As the transcriptome coverage in the target dataset is about 3 X and the contigs
are less than full length, some orthologs may have been missed and the identified targets may be homologous family members.
In addition, clustering followed by assembly of the nucleotide sequences of the 1,818 clones yielded 423 contigs
and 403 singletons.
The purposes of this report are first, to characterize the entire EST collection in terms of contig
number and size, the EST membership of contigs
, and the annotation and putative functional identification of contigs
and singletons; and second, to evaluate a subset of the cDNAs of the collection in regard to their length and coding sequence, the composition of the untranslated region (UTR), and their abundance in the collection.
Together with porcine ESTs (BE014034, DY425421, DY421524), all these fragments were assembled into a 1,913 bp contig
cDNA sequence of pCISH.
Sequence fragments were assembled using the CAP Contig
utility in BioEdit (Hall 1999).
Additionally, the smallest recovered contig
was identical in amino acid sequence to the longer C-tail fragment.
assembled with the minimum number of 2 reads was aligned against the target region reference sequence.
The metrics, such as, N50, longest contigs
and sum of contig
length (total bases) indicate that the present De novo assembly has a good quality (Table 2).
DNA sequences were assembled in a contig
using the SeqMan software.
The variables used as input of ANN for simultaneous estimation of diversity indices were: a categorical index (H': 1 and J: 2) and the number related to the medium (M) and standard deviations (D) of vegetation indices (NDVIM, NDVID, SAVIM, SAVID, EVIM, EVID, MVI5N, MVI5D, MVI7M and MVI7D) and landscape metrics (AREA, GYRATE, SHAPE, CONTIG
, CORE and ENN).