These estimates for DNAm levels were then used for NNLS predictions in independent validation sets, and Epi-Blood-Count results correlated with measurements on a Sysmex XN-9000 hematology analyzer (C; n = 44), on a Coulter counter
(D; n = 24), and microscopic analysis of blood smears and manual counting by a trained operator (E; n = 66).
In case anyone is curious about the sudden science lesson, the patent for the first impedance-based flow cytometer (then called the Coulter counter
) was issued to Wallace H.
The direct counting by Z2 COULTER COUNTER
showed that, in cultures treated with calcimycin, the number of L.
The total number of reticulocytes was then calculated by multiplying the percentage of reticulocytes by the total number of RBC as determined by Coulter Counter
In order to observe the accuracy the counts were also made with Coulter counter
(Beckman Z2) as well as image analysis based counter (BioRad TC-10) with the same bead suspensions of unknown bead number used for micro-capillary bead counter.
For comparative studies, counts were also performed using the Z2 Coulter Counter
instrument according to manufacturer's instructions using the same starting suspension and serially diluted samples.
Assay: To determine the growth inhibitory activity, MDA-MB-453 human breast cancer cells were exposed to increasing concentrations of black cohosh extracts containing 1%, 15%, or 27% triterpene glycosides (Table 1) or purified triterpene glycosides from Cimicifuga species (Table 2) for 96 h and the number of viable cells was determined by the Coulter Counter
Medical professionals quickly established the Coulter Counter
as the most accurate way to count red and white blood cells, which are of different sizes.
After 3 days, one set of cells was harvested and counted at time 0 with a Z1 model Coulter counter
(Coulter Corporation, Miami, FL).