paranense alkaloid fraction (0.01-0.73 mg/ear), solanine (0.001-0.37 mg/ear), or dexamethasone (0.1 mg/ear; used as a positive control) was dissolved in 20 [micro]L of acetone and applied topically before the croton oil treatment.
The ear edema was induced by a unique topical application of croton oil at a concentration of 1 mg/ear in the right ear of the mice and was manifested as an increase of ear thickness .
After 14 days of DMBA application, 1% croton oil
was applied on skin after one hour of palmatine administration three times a week.
Ear edema induced by topical croton oil
: Three groups of six mice were used.
Caption: Figure 9: KCT-01 inhibits ear inflammation induced by croton oil
Mice were anesthetized with ether and 20 [micro]l of an acetone solution containing 0.4 [micro]g croton oil
and appropriate amounts of test samples were applied to the inner surface of the right ear of each mouse.
Groups of mice (n = 8-11) were treated subcutaneously with saline, KPFE (3,10, and 30 mg/kg), KPFV (0.3,1.0, and 3.0 mg/kg), or dexamethasone (2 mg/kg) 30 min before application of croton oil
(2.5% in acetone, 20 [micro]L) on the inner surface of the right ear.
Inflammatory response was induced on the inner surface of the right ear (surface: about 1 [cm.sup.2]) by application of 80 [micro]g of Croton oil
dissolved in an appropriate vehicle, as reported below.
, indomethacin and ursolic and oleanolic acids were Sigma products (Sigma, Milan, Italy).
Hanks balanced salt solution (HBSS), croton oil
, arachidonic acid, dextran, carrageenan, zymosan, plate-let-activating factor (PAF), indomethacin, phenylbutazone, methysergide, dexamethasone, phorbol-12-mirystate-13-acetate (PMA), N-formyl-methionyl-leucyl-phenylalanine (FMLP), sodium nitroprusside (SNP), W-13, H-7, genistein and propidium iodide were purchased from Sigma Chemical Co (USA).
In Group I, 93.75% of animals treated with DMBA and croton oil
developed skin papillomas, and the average number of tumors per mouse (tumor burden) was 6.53.98.
and indomethacin were Sigma products (St Louis, USA).