The disks were removed from the culture tubes
, rinsed with 1x phosphate buffered saline (PBS) to remove any planktonic cells, and transferred to 1.5 mL microcentrifuge tubes containing 1 mL of PBS.
Similarly, we found that a small number of MGIT 960 culture tubes
with an obvious mycobacterial colony in the bottom of the tubes were determined as "culture-negative" by automatic BACTEC MGIT 960 system in the clinical practice (Figure 1).
Pipet 5mL Mueller-Hinton broth into a sterile culture tube
. Inoculate broth with revived cells.
The first ambrosia beetle successfully reared in vitro was Xyleborus ferrugineus Fabricus (Saunders & Knoke 1967), which was reared on a sawdust and agar based medium in glass culture tubes
. Since that time, a variety of ambrosia beetle species have been reared with some success including: Xyleborus pfeili Ratzberg (Mizuno et al.
Because of recruitment of new juveniles (urchins [less than or equal to] 4-mm diameter) smaller than the culture tube
mesh size (4.76 mm), several tubes were found to have higher than 100% survival.
Instead of the glass tube's cylindrical shape, the new plastic culture tube
has a flat side so it will not roll and potentially fall, thereby damaging the graft packaged inside.
During the inoculation of cell monolayers, we placed a negative control using the same cell line injected with maintenance medium after every fifth cell culture tube
. Those negative control cell culture tubes
were also incubated, examined for cytopathic effect, and processed for RT-PCR as for cell culture tubes
injected with specimens.
The upper layer was transferred to a 12 x 75 mm culture tube
and dried under a gentle stream of nitrogen in a 60 [degrees]C water bath.
Experiments were designed to determine the effectiveness of microfuge tubes (1.5 mL) as culture tubes
. Thirty microfuge tubes were loaded with 1 mL of sterile nutrient broth.
After 15 days, data were recorded for percent root induction, number and length of roots per shoot (one explant was inoculated per culture tube
Further, its cost is comparable to the ordinary culture tube
. Therefore, the InPouch culture system may be used as a routine method of diagnosing trichomoniasis.
Under laminar flow hood, the leaves were cut into three pieces using a scalpel and the leaf discs were placed adaxial side down into culture tube
containing 15 ml of MS media.