This is due to generation of toxic chemical residues by Cys-HCl in culture tubes
during late growth phase.
Bring culture tubes
or bottles, stoppers, funnels, and pipettes and all plastic ware into the chamber at least one day before making medium to allow [O.sub.2] to desorb or diffuse out.
containing 5 mL of BHI broth and a cellulose disk were inoculated with ~[10.sup.6] CFU of P.
(2) Collect the filtrate in a sterile culture tube
to obtain spore suspension.
The culture tubes
were incubated at room temperature and MIC was recorded after 24 48 and 72 h (Javaid and Munir 2012).
For in vitro hardening the culture tubes
were kept in a room at ambient temperature (30 [+ or -] 2[degrees]C) and normal day light for 7 days.
Immediately after autoclaving, the media were transferred to a sterile bench and poured into borosilicate glass (18 x 150 mm) culture tubes
(Carolina Biological Supply Co., Burlington, North Carolina), which were then fitted with plastic caps.
Germinated embryos were transferred from petri dishes to glass culture tubes
These different types of explants were transferred separately and individually in culture tubes
containing 20 ml of MS solidified medium  mixed with or without growth regulators.
Quantitative activities of bacteriocin extracts from culture samples of the three LAB under investigation were estimated by using a photometric assay on culture tubes
 (Cabo et al.
The company published an outstanding collection of colored photographs from bacterial culture tubes
to fetal mice, covering most microbiology sub-categories in Schneierson's Atlas of Diagnostic Microbiology (9th edition by 1984).