Cyanine

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Cyanine

 

any one of numerous organic compounds containing two heterocyclic radicals connected by a chain consisting of an odd number of methine groups:

where Y and Y’ are O, S, Se, CR2, or some other element or radical, R and R’ are H or van alkyl, X is Cl, Br, I, or some other anion, and n = 0–5.

The general name for this class of compounds is derived from the first compound of the class, bright blue cyanin, or cyanine blue (from the Greek kyanos, “blue”). Depending on the number of methine groups in the chain, a distinction is made between simple cyanines (monomethines), in which n = 0, carbocyanines (trimethines), in which n = 1, dicarbocyanines (pentamethines), in which n = 2, and so forth. The basic method for the synthesis of cyanines involves the condensation of quaternary salts of heterocyclic compounds. Cyanines are polymethine dyes.

References in periodicals archive ?
The corresponding excitation and emission wavelengths for Cy3 and Cy 5 were 532 and 580 nm, and 633 and 670 nm, respectively.
A team of researchers from Nagoya University and the Japan Science and Technology Agency developed a new probe design based on Cy3 as the fluorophore.
Equal amounts of protein extracts from each sample (total of 6) were pooled, and labeled with Cy2 as the internal standard, while Cy3 or Cy5 were used to label experimental or control samples (Table 1).
Alameda, USA), labeled with Cy3 fluorescent dye, were used (Table 1).
2006) without the permeabilization step, with the following specifications: the primary antibody IgG [alpha] PknG-GST (1:100) and the secondary antibody IgG [alpha]-rabbit conjugated with Cy3 (Chemicon 1:200) were used in cell staining.
On array 1 and array 3, the exercised muscles were incorporated with Cy3 dye, and on array 2 and array 4, exercised muscles were incorporated with Cy5 dye.
leprae strains (One reference strain and another obtained from clinical samples) by comparative genomic hybridization according to the protocol already standardized in our laboratory Cy 5 labelled DNA of standard strain Thai-53 and Cy3 labelled DNA of a lepromatous leprosy patient was used in the microarray experiments for identification of loci showing variability.
For microarray analysis, total RNA was isolated, labeled with Cy3, and hybridized to Agilent Human Whole Genome microarrays.
In cDNA, usually two individual heterogeneous mRNA samples are labelled with either a red-fluorescent dye Cy5 (referred as R) or a green fluorescent dye Cy3 (referred as G), respectively.
Also, a new fluorescence intensity detection cartridge delivers speed and high sensitivity in applications using Cy3 and equivalent rhodamine fluorescent labels.
In the laboratory, each sample is labeled using red Cy5 and green Cy3 dyes.
The use of an array of serial dilutions of the Cy5 and Cy3 dyes with many of the characteristics of an ideal material was explored to measure scanner performance over a 5-week period.