Cyanine

(redirected from Cy5)
Also found in: Dictionary.

Cyanine

 

any one of numerous organic compounds containing two heterocyclic radicals connected by a chain consisting of an odd number of methine groups:

where Y and Y’ are O, S, Se, CR2, or some other element or radical, R and R’ are H or van alkyl, X is Cl, Br, I, or some other anion, and n = 0–5.

The general name for this class of compounds is derived from the first compound of the class, bright blue cyanin, or cyanine blue (from the Greek kyanos, “blue”). Depending on the number of methine groups in the chain, a distinction is made between simple cyanines (monomethines), in which n = 0, carbocyanines (trimethines), in which n = 1, dicarbocyanines (pentamethines), in which n = 2, and so forth. The basic method for the synthesis of cyanines involves the condensation of quaternary salts of heterocyclic compounds. Cyanines are polymethine dyes.

References in periodicals archive ?
The gels were scanned with fluorescence gel scanner, Typoon imager (Trio) (GE Healthcare), using suitable wavelengths and filters for Cy2, Cy3, and Cy5 dyes as per the manufacturer's recommendations The gels images were examined using Progenesis SameSpot software version 3.3 (Nonlinear Dynamics Ltd, Newcastle Upon Tyne, United Kingdom).
Labeling samples GEL 1 Pool (50 [micro]g of proteins +1 [micro]L of Cy2) GEL 2 Pool (50 [micro]g of proteins +1 [micro]L of Cy2) GEL 3 Pool (50 [micro]g of proteins +1 [micro]L of Cy2) GEL 4 Preparative (gel to cut the spots) (500 [micro]g of proteins of all samples applied in the experiment-control and treated triplicates) Labeling samples GEL 1 Control 1 (50 [micro]g of proteins +1 [micro]L of Cy3) GEL 2 Control 2 (50 [micro]g of proteins +1 [micro]L of Cy5) GEL 3 Control 3 (50 [micro]g of proteins +1 [micro]L of Cy3) GEL 4 Labeling samples GEL 1 Treated 1 (50 [micro]g of proteins +1 [micro]L of Cy5) GEL 2 Treated 2 (50 [micro]g of proteins +1 [micro]L of Cy3) GEL 3 Treated 3 (50 [micro]g of proteins +1 [micro]L of Cy5) GEL 4 Table 2 - T.
Of course, the GFP and Cy5 image volumes were already perfectly registered to the episcopic data as they were acquired concurrently.
Briefly, seamless high-resolution images were acquired using an 8-bit monochrome TDI line-image capture camera with filters specific for DAPI to define the nuclear compartment, either fluorescein isothiocyanate (FITC) or Cyanine 3 (Cy3) to define the vimentin-positive tumor cytosolic compartment, and Cy5 to define all target markers.
The peptide was labeled with the NIR fluorophore Cy5 ([[lambda].sub.ex] = 645 nm; [[lambda].sub.em] = 665 nm) and was administered iv for optical imaging of colonic adenomas using a fiber-optic imaging bundle.
(b) The distributions of OVA and CpG in lymph nodes at 8 h after immunization with different vaccine formulations (OVA was labeled with Cy5 and is represented in red; CpG was labeled with FITC and is represented in green; nuclei were labeled with DAPI and are represented in blue; and the colocalization of OVA and CpG is shown in orange).
(C) C labeled with Cy5. The three pictures below: Individual channels of Cy2, Cy3, and Cy5.
The assay consisted of an IC with the Cy5 fluorescence which was able to identify possible RT-PCR inhibition.
The protein of differential group was treated with 200pmol Cy3 (GE Healthcare, 25-8008-61) or Cy5 (GE Healthcare, 25-8008-62) dye markers for comparison on the same gel, and the internal standard was labeled with Cy2 (GE Healthcare, 25-8008-62).
20 [micro]L Cy5.5 dye with five different concentrations of 4, 6, 8, and 10 [micro] mol/L was successively filled into the tube as the fluorescent target.
The device simultaneously captures two different wave lengths emitted by the used fluorochromes (Cy3 and Cy5) and uses the bar code recorded on the rod to determine the specific SNP marker to be evaluated (Figure 1--step 8).