cycloheximide

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cycloheximide

[¦sī·klō′hek·sə‚mīd]
(microbiology)
C15H23NO4 Colorless crystals with a melting point of 119.5-121°C; soluble in water, in amyl acetate, and in common organic solvents such as ether, acetone, and chloroform; used as an agricultural fungicide.
References in periodicals archive ?
Cyclohexamide: inhibition of Dermocystidium marinum in laboratory stocks of oysters.
Cultures were maintained at 37[degrees]C and 5% C[O.sub.2] in chlamydial growth medium (CMGH), which contains minimal essential medium (MEM; Cellgro, Mannassas, VA, USA]; 10% fetal bovine serum (FBS; University of California, San Francisco [UCSF] Cell Culture Facility, San Francisco, CA, USA); 0.45% glucose solution (Cellgro); 20 mmol/L HEPES (UCSF Cell Culture Facility); 0.08% NaHC[O.sub.3], 10 [micro]g/mL gentamicin (MP Biomedicals, Solon, OH, USA); 25 [micro]g/mL vancomycin (Acros Organics, Morris Plains, NJ, USA); 25 units/mL nystatin (MP Biomedicals), 375 [micro]g/mL amphotericin B (PharmaTek, Huntington, NY, USA); 1 [micro]g/mL cyclohexamide for 48 h and harvested as described (18,19).
After centrifugation, the inocula were removed and replaced with CMGH plus 1 [micro]g/mL cyclohexamide and maintained at 37[degrees]C in 5% C[O.sub.2].
trachomatis serovar LGV-L1 and LGV-L2 were propagated in a semiconfluent McCoy cell sheet for 48-72 h in EMEM media with 10% v/v FCS and cyclohexamide [2ug [mL.sup.-1]].
The filters were incubated on plates of BG11 media [24] containing cyclohexamide (100 ug/mL) for two weeks at 25 +- 2 AoC, illuminated by continuous 18 umol photons m-2 s-1 given by a white fluorescent tube light in a controlled environmental growth chamber (Sanyo versatile MLR-350H).
Finally purified colonies or filaments were transferred to BG11 broth without cyclohexamide for further experiments.
Second swab was used for streaking Blood agar (BA), Chocolate agar (CA) and Mac Conkey agar (MA), third one for KOH; forth one to streak Sabarauds Dextrose agar without cyclohexamide.
By investigating the effects of high pC[O.sub.2] on hepatocytes from Antarctic fishes incubated with the protein synthesis inhibitor cyclohexamide, Langenbuch and Portner (2003) demonstrated that protein biosynthesis was impaired by cellular acidosis.
We have not performed experiments in the presence of cyclohexamide.