Cytological Diagnosis

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Cytological Diagnosis


a method of diagnosing diseases and studying the physiological condition of a human organism based on the investigation of cell morphology and cytochemical reactions.

Cytological diagnosis is used in various branches of medicine. In oncology it is used to diagnose malignant and benign tumors, in mass screenings to detect the early stages of cancer and pre-cancerous diseases, and to observe the course of antitumor treatment. In hematology it is used to diagnose various diseases and evaluate therapy effectiveness, and in gynecology, to diagnose various types of cancer and to determine pregnancy and hormonal disturbances. It is also used to diagnose many diseases of the respiratory, digestive, urinary, and nervous systems and to evaluate the results of therapy.

Cytological examination is valuable in diagnosing various types of malignant tumors, in assessing the extent of malignancy, and in determining the type of tumor tissue involved; the histological form and size of a tumor are established in 70–85 percent of the cancer cases. Various criteria have been developed for the cytological diagnosis of diseases of the hematopoietic and reticulo-endothelial systems, some diseases of the stomach and kidneys, tuberculosis, skin diseases, and a number of other diseases. When required, cytological diagnosis can be made quickly. Cytological methods are often combined with histological methods.

Cells for examination are obtained by a variety of methods. In the exfoliative method, cells that are sloughed off naturally in the normal fluids of the body, such as blood, are studied, as well as cells that are found in pathological discharges, such as sputum, or that are artificially separated by washing and desquamation. In another method, the material is extracted with a fine needle through a puncture (exploratory puncture). The introduction of endoscopy into clinical practice has led to the widespread use of the biopsy method of cytological diagnosis.

The specimen is prepared on slides for cytological examination. Various techniques are used, depending on the purpose of the examination, for example, microscopy of material in the normal state or of fixed and stained material, using standard staining techniques, and phase-contrast microscopy, ultraviolet microscopy, and fluorescence microscopy (with fluorochromes used to stain the preparations). A number of cytochemical methods have found practical applications. Special methods are also used in research, including autoradiography, immunocytochemistry, spectrophotometry, fluorimetry, electron microscopy, and tissue culture.


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Henning, N., and S. Witte. Atlas der gastroenterologischen Zytodiagnostik. Stuttgart, 1968.


References in periodicals archive ?
Considering the cytological diagnosis, the three most frequent HPV genotypes were 16,18, and 51 in AGUS, ASCH, LSIL, HSIL, while in cancer and ASCUS the type 58 was the third most frequent, behind genotypes 16 and 18.
Nearly 80% (82/104) of effusions reported by cytological diagnosis as MM had elevated mesothelin levels (Figure 7(b)).
Histopathology Total Correlation with number cytological diagnosis of cases Accurately Diagnosed as categorized benign but not accurately categorized Adipocytic tumors 68 68 0 Fibroblastic tumors 10 2 8 Fibrohistiocytic tumors 11 6 5 Smooth muscle tumors 2 0 2 Pericytic tumors 1 0 1 Vascular tumors 24 23 1 Chondroosseous tumors 1 0 1 Peripheral nerve sheath 23 4 19 and related tumors Total 140 108 (77.14%) 32 (22.86%) TABLE 4: Correlation of cytodiagnosis and histopathological diagnosis in malignant tumors.
A cytological diagnosis of spindle cell lesion of neural origin with the closest resemblance of ectopic meningioma was rendered.
The accuracy of cytological diagnosis depends on several factors including the experience of aspirator, sampling method, the adequacy of sample the target organ and the expertise of examiner (Cytotechnologist and cytopathologist).
The cytological diagnosis of smears distribution was: 1120 (75.5%) were diagnosed as LSIL, 354 (23.9%) were HSIL and 9 (0.6%) ASC-US (Table I).
The participants were included following a consecutive order by using the selection criteria previously established such as a previous cytological diagnosis realized by the specialized physician and without histerectomy.
The cytological diagnosis of 378 cases include, 340 cases benign, 10 cases suspicious,18 cases malignant, and 10 cases inadequate (Table 1);.
These findings supported the initial cytological diagnosis of small cell carcinoma.
Ultrasonography guided needle aspiration yielded a cytological diagnosis of the hematoma.
Of particular concern are the findings that almost 10% of patients with high-grade squamous intraepithelial lesion (HSIL) Pap smear findings had only repeat smears as follow-up within 1 year and that almost 9% of patients with a cytological diagnosis of cancer had another Pap smear performed as the initial follow-up procedure.
In a previous retrospective study (15), our group showed that K-ras mutation analysis in paraffin-embedded FNA samples from pancreatic masses contributed to cytological diagnosis in a substantial proportion of cases, mainly when suspicious cells, healthy-appearing duct cells, or insufficient material was reported, without false-positives cases.