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SUITABILITY OF THE DASL ASSAY TO DETERMINE GENE EXPRESSION PROFILES OF EPITHELIAL CELLS SPIKED AT LOW FREQUENCIES IN HDB AND EFFICACY OF AdnaWash BUFFER
To evaluate the reliability of the DASL assay for obtaining gene expression profiles (GEPs) from both low RNA input samples and CTCs, experiments were run with low predefined numbers of breast cancer cells (MCF7 and MDA-MB-468), spiked into a HDB at final concentrations of 400, 200, and 100 cells/5 mL.
The DASL data and story "library" was created as part of a larger effort to enhance the teaching of statistics using computers, and is one of the many data links suggested on the Graph Master resource webpage (above).
Michael O'Dwyer, DASL Newham Community Drug & Alcohol Team Senior Manager, explains: “DASL works alongside a range of external organisations, including health and social care providers.
We recognise that data security is going to be on the agenda for many organisations in 2014, particularly when it involves vulnerable individuals, so it is great to see charities such as DASL taking proactive steps to secure the information they share.
For each sample, 200 ng of total RNA was converted to cDNA and processed in the DASL assay as described previously (11,13).
Illumina's DASL Assay was specifically designed to successfully profile expression in degraded RNA, such as that found in formalin-fixed paraffin embedded (FFPE) samples.
Illumina's DASL assay opens up a new avenue for reproducible RNA profiling at high multiplex and at low cost per sample.
Supports SOAP, WebDAV, DASL, and Delta-V protocols as well as communications via Java applets, Javascript, and VBScript
The DASL portion is a parametric or cause-and-effect study designed to expose various diesel emissions control systems to oil combustion byproducts.
The group's collective efforts lasted more than two years and resulted in the WebDAV standard, along with a series of WebDAV-related specifications, including DASL and Delta-V.
The latest version of PortalWare also supports additional Web standards such as WIDL (Web Interface Definition Language) for passing data between applications and DASL (DAV Searching and Locating) for advanced searching of web-based content collections.
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