DEAE-cellulose


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DEAE-cellulose

[′dē‚ē′ā‚ē ′sel·yə‚lōs]
(organic chemistry)
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Fractionation of the crude SP by anion-exchange chromatography on a DEAE-cellulose column using a stepwise of NaCl (0[right arrow]1 M, with 0.25 M intervals) was performed and -SP1, -SP2- and Cc-SP3 (eluted with 0.5, 0.75, and 1 M NaCl, respectively) were obtained.
The substrate L-asparagine, DEAE-cellulose and Sephadex G-100 were procured from Sigma (Sigma-Aldrich, USA).
Kayastha, Immobilization of Pigeonpea (Cajanus cajan) Urease on DEAE-Cellulose Paper Strips for Urea Estimation, Biotechnol.
Trypsin inhibitor purification was done by ion-exchange chromatography on DEAE-cellulose, gel-filtration - on Sephadexes G-15 and G-50, by affine chromatography on trypsin - sepharose 4B.
Sequential elution of denatured proteins, hydrolyzed RNA, and plasmid DNA of bacterial lysates adsorbed onto stacked DEAE-cellulose membranes.
Glucose-6-phosphate (G6P), [beta]-Nicotinamide adenine dinucleotide phosphate ([NADP.sup.+]), [beta]-Nicotinamide adenine dinucleotide phosphate, reduced (NADPH), 6-phosphogluconic acid (6PG), adenosine-57-triphosphate (ATP), adenosine diphosphate (ADP), [beta]-Nicotinamide adenine dinucleotide (NAD), [beta]-Nicotinamide adenine dinucleotide, reduced (NADH), DL-Dithiothreitol (DTT), nitroblue tetrazolium (NBT), phenazine methosulphate (PMS), blue dextran, bovine serum albumin (BSA), Sephacryl S-300, molecular weight marker kits for gel filtration, diethylaminoethyl-cellulose (DEAE-Cellulose), and chemicals for electrophoresis and isoelectric focusing (IEF) standard markers mixture pi 3.6-9.3 were purchased from Sigma Chemical Co.
Protein content Total activity Specific (mg) (titer/mg x activity [10.sup.3]) Crude 3200 384 120 Ion exchane on 1600 256 160 chromatography DEAE-cellulose Affinity 20 51 2550 chromatography on glucose-sepharose Hemagglutination activity
Protease inhibitors were extracted from the seeds of Lavatera cashmeriana Camb by ammonium sulphate precipitation and purified by chromatography on DEAE-cellulose and Sephadex G-100 column.
Characterization of the steps of amine oxidase purification process from pea seedlings Fraction Total Specific Recovery, activity, activity, % IU IU/mg Centrifuged homogenate 84.55 0.51 100 Ammonium sulphate fractionation (30%) 79.40 0.52 93.9 Ammonium sulphate fractionation (70%) 68.66 0.75 81.2 DEAE-cellulose column 48.44 0.80 57.3 Sephadex G-200 column 43.83 0.83 51.8 [TABLE 2 OMITTED]
in skin care products, such as physical adsorption on chitin, ionic binding onto DEAE-cellulose, covalent bonding on CNBr-Agarose and entrapment in alginate.
Activity of cellulases was tested in the protein dialysate and was further purified by DEAE-Cellulose column chromatography.