Biologic and hybridization replicates generated statistically significant information pertaining to the expression pattern of all 1,700 genes on the DNA chip
. Clustering analysis and statistical correlation revealed that gene expression profiles produced in animals treated with different agents from a common class of compounds (peroxisome proliferators) were similar.
10, 1999 SCIENCE illustrated the promise of DNA chips
in fruit fly research.
This could be a great advantage for low-cost automation in fabricating high-degree multiplex DNA chips
In all, 53 samples failed initial analysis in the Apples series and required repeat analysis compared with 54 samples in the CF Portrait DNA Chip
series, yielding an acceptable repeat rate of ~5% for both platforms.
Fodor, president of Affymetrix, a firm in Santa Clara, Calif., that has been at the forefront of DNA chip
manufacturing and research.
co-developed that technology with the Research Institute of Genome-Based Biofactory of the National Institute of Advanced Industrial Science and Technology.
In addition, DNA Chip
Research has concluded a licensing agreement with SIGMA-ALDRICH Japan.
Genetics Institute, Cambridge, Mass., and Affymetrix also recently announced an expanded program for development of gene expression monitoring chips based on GeneChip technologies, following completion of their initial three-year collaboration demonstrating innovative use of DNA chips
for gene expression monitoring.
In each of the above examples as well as in other versions of microaddressable array technologies, the reliance on an optical detection system to readout the DNA chip
necessitates using large and expensive instrumentation.
The genotypes scored by the polypyrrole DNA chip
assay were 100% in agreement with conventional DNA sequencing results.
The dispute arose from Affymetrix' purchase of Beckman Coulter's gene chip business in 1999 and if the purchase gave Affymetrix rights to DNA chip
technology licensed to Beckman Coulter by OGT.
The second type of DNA chip
is used to determine gene expression profiles for cells or tissues, either as differential expression between two cell types or expression for a single cell type.