Cloning and expression in escherichia coli of the ogg1 gene of saccharomyces cerevisiae, which codes for a DNA glycosylase
that excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-n-methylformamidopyrimidine.
SLE: systemic lupus erythematosus; DNA: deoxyribonucleic acid; 8-oxodG: 8-hydroxy-2'-deoxyguanosine; hOGG1: 8-oxoguanine DNA glycosylase
; PARP: poly-ADP ribose polymerase; XRCC1: X- ray repair cross-complementing protein 1; ANAs: antinuclear antibodies: Pol polymerase beta; CPDs: pyrimidine cyclobutane dimers; 6-4 PPs: 6-4-pyrimidine pyrimidone photoproducts; UV: ultraviolet radiation; XPA: xeroderma pigmentosum complementation group A; XPC: xeroderma pigmentosum complementation group C; XPE: Xeroderma pigmentosum complementation group E; DDB1 DNA damagebinding protein 1.
The labeled oligonucleotide (2.5 nM) and 300 fmoles of each DNA glycosylase protein were incubated in 20 [micro]L of the reaction mixture, which was described previously , at 37[degrees]C for the indicated time, and the mixture was then treated with 0.1 N NaOH.
Next, we attempted to investigate whether DNA glycosylase proteins are involved in the repair of 8BrG.
Human DNA glycosylases involved in the repair of oxidatively damaged DNA.
The DNA glycosylases OGG1 and NEIL3 influence differentiation potential, proliferation, and senescence-associated signs in neural stem cells.
Further, this compaction has been shown to reduce recruitment of the DNA glycosylase
Ogg1, delaying the repair of oxidatively induced DNA lesions (Amouroux et al.
Panayotou et al., "Crystal structure of a G:T/U mismatch-specific DNA glycosylase: mismatch recognition by complementary-strand interactions," Cell, vol.
Drohat, "Crystal structure of human thymine DNA glycosylase bound to DNA elucidates sequence-specific mismatch recognition," Proceedings of the National Academy of Sciences of the United States of America, vol.
Cloning and characterization of a mouse 3-methyladenine/7-methylguanine/3-methylguanine DNA glycosylase
cDNA whose gene maps to chromosome 11.
Thus, our results suggest that the abnormal regulation of NEIL1, NEIL2, and NEIL3 expression is involved in the development of cancer via an increase in the prevalence of somatic mutations, providing a new and important link between abnormalities in the DNA glycosylases
NEIL1, NEIL2, and NEIL3 and human cancer.
Sossou et al., "Role of XRCC1 in the coordination and stimulation of oxidative DNA damage repair initiated by the DNA glycosylase
hOGG1," The Journal of Biological Chemistry, vol.