ligase

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ligase

[′lī‚gās]
(biochemistry)
An enzyme that catalyzes the union of two molecules, involving the participation of a nucleoside triphosphate which is converted to a nucleoside diphosphate or monophosphate. Also known as synthetase.
References in periodicals archive ?
In the paper, according to RSAS, "Modrich demonstrated the requirement of DNA polymerase III, exo-nuclease I, and DNA ligase for mismatch repair.
The recent observations that DNA ligase III levels are decreased in the absence of ATM may also explain these results (Sharma et al.
1997) Mammalian DNA double-strand break repair protein XRCC4 interacts with DNA ligase IV.
Then, the purified PCR product was ligated to pMD18-T vector (an T cloning vector) by T4 DNA ligase at 16 oC overnight.
05 [micro]l (40 U/[micro]l) ligase (Taq DNA ligase enzyme system), 1 [micro]l PCR product (100 ng/[micro]l), and 6.
Note exquisite sensitivity in cell strains derived from DNA damage sensing- or repair-deficient cells derived from patients with abnormal ATM, NBS1 and DNA ligase IV proteins.
In the blunt end, a DNA ligase will join together the 3' end and the 5' end of one molecule with the 5' end and the 3' end, respectively, of another molecule.
The insert and vector were then ligated using T4 DNA ligase following the manufacturer's protocol.
Ligation was carried out using T4 DNA Ligase (Promega) in a 4[degrees]C overnight reaction.
The ligation mixture consists of 1 [micro]l of pUC19 vector, 9 [micro]l of 2x ligation buffer, 1 [micro]l of T4 DNA ligase, 4.
Each ligation reaction (20 [micro]L) contained the restriction digestion product (10 [micro]L), 1 x T4 DNA ligase buffer with EDTA, 0.