DNase

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Related to DNAase: Dnase 1

DNase

(biochemistry)
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Positive results were recorded for methyl red test, voges proskauer and catalase test while negative test for DNAase test.
Serological Tests: (ASO, Antistreptokinase, Antihyaluronidase, Anti DNAase B, Anti NDAase) Most of these tests assay for neutralizing antibodies to streptococcal extra cellular enzymes.
A 400-[micro]L plasma sample was first lysed using the Qiagen protease, which is free of DNAase and RNAase activity, and with buffering conditions (salts and pH) that allow the binding of only DNA to the QIAamp membrane, excluding proteins and other contaminants that can inhibit PCR.
Total RNA for 3rd-instar larva, pupa and adult was extracted using Trizol (Transgen, Beijing, China), and treated with RQ1 DNAase (TaKaRa, Dalian, China) to exclude gDNA.
Besides these snakes venom contains enzymes viz hyaluronidase, phospholipase A, phospho diasterase, choline estarse, DNAase, RNA ase etc.
The isolates were identified by colony morphology, Gram stain, motility, catalase, coagulase, DNAase, sugar fermentation and oxidase tests.
PCR products each of them has 50[micro]l volume containing 26[micro]l PCR master kit, 1[micro]l DNA, 3[micro]l from each of the forward and reverse primers (with 10pmol density, each) and 17[micro]l DNAase free water, were prepared and tested with the 1.
So, they used the enzyme DNAase together with negatively charged poly aspartic acid to break down the chemical bonds of these elements that support the biofilm.
The results obtained contributed strong evidence that the non-disjunction would compromise extensive regions of the telomeric portion of the q arm of chromosome 21; these were characterized for having high-density zones of CpG islands, portions of chromatin sensitive to treatment with DNAase I, with a variable degree of methylation of some residues of lysines from the histone H3; besides having a high intensity of recombination in regions coinciding with a high degree of polymorphisms by deletions and insertions.
To work with the mRNA for sensitive procedures like RT-PCR, it is recommended to either purify the Poly A RNA by chromatography on oligo (dT) cellulose (as described in the method of Blumberg and Lodish) or usage of DNAase to remove trace amounts of DNA (the kit methods).