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faecalis suspensions were mixed with equal volumes of DNase I (final concentration 50 Kunitz/mL; 5 mM [MgCl.sub.2] in 0.9% NaCl) or heat-inactivated DNase I (100[degrees]C, 30 min) and injected into the root canals (n=56) using sterile syringes (gage 27, BD Microlance, Franklin Lakes, NJ, USA).
Thereafter, teeth were carefully dried with sterile paper points, and DNase I or heat-inactivated DNase I (50 Kunitz/mL; 5 mM [MgCl.sub.2] in 0.9% NaCl) was injected with sterile syringes and left to incubate for 1 h at 35[degrees]C under anaerobic conditions.
In addition, the lung wet/dry ratio declined partially in the DNase I pretreated group compared with the vehicle group (Figure 5(a)).
Recombinant human DNase I has been developed clinically for treatment of pulmonary disease in patients with cystic fibrosis [35].
A DNase I high concentrate stock solution was produced at 40 mg/mL using DNase I and nuclease-free water.
When the number of viral particles in the sample was high, we omitted the RNase A and DNase I treatments and used the RNeasy Mini Kit (Qiagen) for RNA extraction.
Deoxyribonuclease I (DNase I; EC 3.1.21.1) is genetically polymorphic (1), and has been postulated to be a candidate molecule for the endonucleolytic activity involved in apoptosis or programmed cell death (2) and involved in the initiation of systemic lupus erythematosus (3).
One of them, an enzyme called DNase I, appears less active in many lupus patients than in healthy people.
Crystal found that twice-daily inhalations of the drug, called DNase I, improved the lung functions of all 16 of the adult subjects.
DNAse is robust enough that it works in this procedure, even without the included buffer.
DNase is not as prevalent as RNase, but it is a concern of molecular biologists especially those doing polymerase chain reaction (PCR) with very small quantities of rare DNA.