Inhibiting capacity of hydroxyl radicals by phytic acid using the Deoxyribose
* RNA contains a ribose sugar hydroxylated at the second carbon, while DNA contains a deoxyribose
sugar protonated (hydrogen atom) at the second carbon
One of the preferred targets for [H.sub.2][O.sub.2] is the DNA; it produces single- or double-stranded DNA breaks as well as DNA cross links, in addition to purine, pyrimidine, or deoxyribose
assay was carried out essentially, as described by Halliwell et al.
Halliwell, "The deoxyribose
assay: an assay both for 'free' hydroxyl radical and for site-specific hydroxyl radical production," Biochemical Journal, vol.
Folin-Ciocalteu reagent, sodium carbonate, gallic acid, rutin, aluminium chloride, sodium nitrate, sodium hydroxide, 2,2-diphenyl-1-picrylhydrazyl (DPPH), trichloroacetic acid, potassium ferricyanide, sodium acetate buffer, neocuproine, deoxyribose
, EDTA, potassium phosphate buffer, hydrogen peroxide, ascorbic acid, TBA, 2,4,6 tripyridyl-s-triazine (TPTZ), ferric chloride, HCl, ammonium molybdate, sodium phosphate, sulfuric acid, ammonium thiocyanate, and all other chemicals used were of analytical grade.
radicals, brain peroxidation and degradation of benzoate, deoxyribose
, amino acid and DNA.
ROS-induced DNA damage can result in DNA single- or double-strand breakage, base modifications, deoxyribose
modifications, and DNA cross-linking.
celtidifolius showed antioxidant properties, due to scavenged superoxide anions, inhibited deoxyribose
degradation and lipidic peroxidation in vitro, and antiedematogenic effects by reducing paw edema induced by carrageenan in rats (Nardi et al., 2003).
Free radicals can attack DNA at C4 of deoxyribose
generating products as propenal, which react with 2-thiobarbituric acid and produce the TBARS formation .
Hydroxyl radical was produced in the presence of 20 [micro]M Fe[Cl.sub.3], 1.4mM [H.sub.2][O.sub.2], 2.8 mM deoxyribose
, 100 [micro]M EDTA, and 100 [micro]M ascorbate, in 10 mM phosphate buffer, pH = 7.4, as was published earlier (Sanz et al., 1994).
For antioxidant analysis, reagents were used as follows: dimethyl thiourea (DMTU), nordihydroguaiaretic acid (NDGA), ascorbic acid, histidine, xylenol orange, ammonium iron (II) sulfate hexahydrate, N,N-dimethyl-4nitrosoaniline (DMNA), catalase, xanthine, xanthineoxidase, nitroblue tetrazolium (NBT), deoxyribose
, and butylated hydroxytoluene (BHT) were from Sigma Aldrich (St.