(2) The electrochemical polymerization of 4-carboxyphenyl diazonium salts prepared by the diazotization
reaction is a very simple and easy method for preparation of good biosensor supports.
5-phenyl-1,3,4-thiadiazole-2-amine was synthesized by single step reaction and it was transformed to its corresponding diazonium salt by diazotization
reaction and was further coupled with various coupling agents (8-hydroxy quinoline, 2,6-diaminopyridine N,N-dimethyl aniline, 2-naphthol, resorcinol, and 4,6-dihydroxypyrimidine) under suitable experimental reaction.
Indirect flow injection voltammetric determination of sulphanilamide by monitoring at a glassy carbon electrode the excess of nitrite remaining after its diazotization
has been reported by Fogg et a1 .
The absorbance of the chromophore formed during diazotization
of the nitrite with sulphanilamide and subsequent coupling with N-1-naphthylethylene diamine dihydrochloride was measured at 546 nm and percentage scavenging activity was measured with reference to standard.
Methods: Partially purified Amaranthus spinosus leaf oxalate oxidase was immobilized through diazotization
onto arylamine glass beads affixed on the surface of a plastic strip by a non reactive fixative and employed for oxalate determination in urine and serum samples collected from healthy individuals and urinary stone formers.
Nitrite level was determined by using diazotization
Bilirubin was measured on the Modular analyzer by a modified diazotization
method using 2,5-dichlorophenyldiazonium tetrafluoroborate as the source of diazonium ion (Roche Diagnostics Corporation).
Ammonium-N ([NH.sub.4]-N) concentrations (by reaction with salicylate/nitroprusside) and [NO.sub.3]-N concentrations (by diazotization
following hydrazine reduction) were measured on a Technicon AutoAnalyzer II system (Blakemore et al.
of the amine produces a diazonium intermediate that is not isolated but is reduced with tin chloride to the corresponding hydrazine.
Concentrations of ammonium nitrogen ([NH.sub.4]-N) were determined using the phenol blue method, and concentrations of nitrate nitrogen ([NO.sub.3]-N) were determined by diazotization
with sulfanilamide after reduction of nitrate to nitrite through a cadmium coil (Keeney and Nelson 1982).
Ammonium (salicylate/nitroprusside; Keeney and Nelson 1982) and N[[O.sub.3].sup.-] (diazotization
following cadmium reduction; Keeney and Nelson 1982) were determined using an Alpkem RFA 300 Rapid Flow Analyzer (Clackamas, Oregon, USA).
Nitrate was reduced to nitrite using hydrazine sulphate and CuS[O.sub.4] catalyst and then analyzed colorimetrically by the Giess-Ilosvay reaction (diazotization