This method, originally applied to the process of differential extraction
of male/female DNA from a mixed forensic sample, has broad application in a wide variety of biological sample processes for analysis and identification.
This procedure is known as differential extraction (Gill et al.
At the same time, a robotic liquid handler will be programmed to execute a proprietary differential extraction procedure that is based on differential lysis and selective filtration.
The more we use the unit and explain its operation to our clients--and show them the "before" and "after" comparisons--the greater is our level of comfort in setting reasonable standards for how-clean-is-clean, Correlation of the ATP readings with CFUs has been attempted by various researchers, but it must be re-evaluated with each circumstance and is a complicated procedure involving filtration of the samples and differential extraction
of somatic ATP and microbial ATP.
Promega announces the new automated capabilities of the Differex[TM] Differential Extraction System.
By contrast, the automated Differex method makes it possible to process up to 40 differential extraction samples in less than 5 hours, reducing total processing time by over 70%.
Currently, the laboratory procedure to perform this tedious DNA processing technique, referred to differential extraction
, involves numerous laboratory-based, labor-intensive techniques that are subject to contamination and human error.
This patent, entitled "Absorption-Enhanced Differential Extraction
Device", and developed by researchers at the University of Washington and at Micronics, Inc.
In Case 2, using the differential extraction protocol, stains N1, N2, and N3 gave no autosomal STR or Y-STR results.
The fact that no sperm cell was visibly detected from the Christmas Tree Staining Test and that the results from the differential extraction protocol were negative, the Y-STR results obtained from stains N1 and N2 could have originated from underlying traces of his epithelial, saliva, or other cells.
This patent, entitled Microfabricated Differential Extraction
Device and Method, covers key technologies that Micronics is commercializing.
In rape cases, when the presence of semen, sperm, or both is identified, a differential extraction
of the sample is done to separate the sperm from epithelial cell DNA.