Bio-Rad's iQ-Check real-time PCR test kits were selected based on their proven real-time PCR technology that uses highly specific patented DNA probes
to detect pathogenic bacteria in a sample.
The HPLC-purified DNA probes
and RNAs are synthesized by Sangon Biotech (Shanghai, China) Co., Ltd.
Nine kinds of DNA probes
for miRNA testing are synthesized and fixated on the corresponding coding microspheres to become a suspension array.
Panel 1 contained column-based individually synthesized DNA probes
, affording individual re-synthesis should quality control checks identify any stochastic failures.
and PCR primers for the detection of a phytoplasma associated with peanut witches'-broom.
Coating of oxidized agarose on glass slides can provide aldehyde groups which bind to amino-modified DNA probes
(19), also DNA probes
can attach to agarose-coated glass surface using ultraviolet (UV) radiation (19), (20).
In principle, DNA probes
can be used instead of RNA probes to detect mRNA transcripts.
According to the paper, the tumour marker testing is a major opportunity for DNA probes
, particularly for the application of molecular pathology technologies.
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes
. Proceedings of the National Academy of Sciences, USA, 74, 53 5 0- 53 54.
Then the researchers designed a microarray of more than 700 DNA probes
to detect the resistant genes.
FISH detects and quantifies abnormal cells by using dye-labeled DNA probes
of cell chromosomes that cause cells with the targeted genetic abnormalities to light up when viewed under a fluorescent microscope."We were surprised to find many more abnormal circulating cells in lung cancer patients compared with what had been seen previously using other techniques," Katz said.The researchers used 12 biomarker probes that target aberrations previously connected to lung cancer to analyze 59 cases of non-small cell lung cancer and 24 controls, people without lung cancer, including smokers and non-smokers.