Dounce homogenizer

Dounce homogenizer

[′dau̇ns hə′mäj·ə‚nīz·ər]
(biology)
An apparatus consisting of a glass tube with a tight-fitting glass pestle used manually to disrupt tissue suspensions to obtain single cells or subcellular fractions.
References in periodicals archive ?
Frozen human cardiac muscle was weighed and homogenized using a small glass Dounce homogenizer (D8938, 2 mL; Sigma Aldrich) at room temperature in different volumes of pooled human serum with a free [Ca.sup.2+] concentration of 1.25 mmol/L at the ratios specified in Fig.
Lesion crusts and biopsy material may be soaked in 3 volumes buffer and solubilized by 10-12 pestle strokes in a Dounce homogenizer, while fecal material may be suspended by vortexing with glass beads in 3-9 volumes of distilled water.
At targeted stages of development, embryos were frozen, rinsed twice in cold tank water, dissolved in 1 mL of TRI REAGENT, and transferred to a Dounce Homogenizer (Fisher Scientific, Pittsburgh, PA), previously washed with chloroform.