Dounce homogenizer

[′dau̇ns hə′mäj·ə‚nīz·ər]

(biology)

An apparatus consisting of a glass tube with a tight-fitting glass pestle used manually to disrupt tissue suspensions to obtain single cells or subcellular fractions.

References in periodicals archive ?

Frozen human cardiac muscle was weighed and homogenized using a small glass Dounce homogenizer (D8938, 2 mL; Sigma Aldrich) at room temperature in different volumes of pooled human serum with a free [Ca.sup.2+] concentration of 1.25 mmol/L at the ratios specified in Fig.

Revision of the troponin T release mechanism from damaged human myocardium

Lesion crusts and biopsy material may be soaked in 3 volumes buffer and solubilized by 10-12 pestle strokes in a Dounce homogenizer, while fecal material may be suspended by vortexing with glass beads in 3-9 volumes of distilled water.

Electron microscopy for rapid diagnosis of infectious agents in emergent situations (1). (Synopses)

At targeted stages of development, embryos were frozen, rinsed twice in cold tank water, dissolved in 1 mL of TRI REAGENT, and transferred to a Dounce Homogenizer (Fisher Scientific, Pittsburgh, PA), previously washed with chloroform.

Green fluorescent protein (GFP) as a marker of aryl hydrocarbon receptor (AhR) function in developing zebrafish (danio rerio). (Articles)