During the development of our ELISA assay
, we found that the highest signal was obtained in a solution containing 2 mmol/L calcium, with a polyclonal goat antihuman Gas6 antibody for the capture antibody and a biotinylated polyclonal goat antihuman Gas6 antibody for the detection antibody, visualized via an avidin-coupled enzymatic reaction.
Only one of the eight serum samples showed a very low but detectable TK activity (92 [micro]IU/L) when the AZT substrate was omitted, and this was a false-positive signal, of unknown origin, in the TK ELISA assay
However, protein concentrations measured by immuno-LCMS/MS were much higher than expected based on the observed population range measured with the commercial ELISA assay
. As expected, comparison of Lp-[PLA.sub.2] concentrations measured by immuno-LCMS/MS and immunoassay showed a similar discrepancy between the 2 methods (Fig.
Multi-country evaluation of the sensitivity and specificity of two commercially available NS1 ELISA assays
for dengue diagnosis.
Caption: Figure 2: Standardization of the sample volume for use in the IgG DENV ELISA assay
. Nine serum positive samples (red) and three negative samples (blue) were tested.
Western blotting and ELISA assay
were performed for identification and confirmation of immunoreactivity of produced rPR.
Relative CD36 expression in 3-D and 2-D cultures was measured with an ELISA assay
specific to extracellular CD36 proteins (Figure 4).
Here, using real-time PCR and ELISA assay
, we found that compared with control siRNA cells the expression and secretion of MMP-7 were greatly suppressed in radixin siRNA cells (Figures 6(a) and 6(b)).
Gennova-PictArrays require only 50 [micro]L of a 200-fold diluted sample and follows the same steps as a traditional ELISA assay
. Together with low setup costs and a fast sample processing time, Gennova-PictArrays provide an affordable alternative to currently available multiplex testing systems.
Unfortunately, the ELISA assay
that we have now for NoV detection is relatively insensitive, and most clinical laboratories cannot perform RT-PCR.
HAAH levels are measured using an ELISA assay
. In a double blind study of 857 samples, conducted previously by Panacea, the test showed 94.7% sensitivity, 94.3% specificity and an overall accuracy of 94.6%.
Other examples of autoimmune products and services from the company include: a mix of anti-collagen monoclonal antibodies; readily available ELISA assay
kits and custom development of diagnostic assays.