Colonies showing metallic shine on EMB agar
were suspected for E.
is used specifically for the growth of coliforms that are disease causing agents in fish and also in humans.
coli Isolates The colony appeared as green with black metallic sheen of the stool samples which were cultured on Eosin Methylene Blue agar, and again were picked and sub cultured on fresh EMB agar
plates to obtain presumptive E.
medium###sheen colonies###center###colonies###point like###colonies
The isolates were identified based on colony characteristics and biochemical profile including oxidase, indole production, methyl red, VP, citrate utilization, glucose fermentation, lactose fermentation, Na requirement, tripple sugar iron, motility tests and growth on EMB agar
Samples were cultured on blood agar & EMB agar
. Colony counts of more than 104 cfu/mL were considered positive.
Para a confirmacao de coliformes fecais todos os tubos que apresentaram turvacao e producao de gas foram repicados para placas contendo EMB Agar
(Teague) HIMEDIA[R] e incubadas a 45 [degrees]C durante 24h.
Selected colonies were identified by gram staining .Growth patterns were observed using EMB agar
and KKA agar.
In order to test this, goose fecal, influent, effluent, and river water samples were collected and isolated by being streaked onto EMB agar
. The E.
The loopful of culture, from the positive presumptive tube was streaked on sterilized EMB agar
Appropriate dilutions were prepared and 0.1 mL of each dilution was spread plated on EMB agar
. The detection limit was determined to be 1 log CFU/[cm.sup.2] (i.e., 10 CFU/[cm.sup.2]) to avoid the introduction of error into the results.