Table-I: Characteristics of patients screened for ESBL
production was confirmed by increase in zone diameter of more than 5mm of ceftazidime towards clavulanic acid.12 DDST was done by using five antimicrobials, namely amoxicillin-clavulanic acid having quantity of 20mcg and 10mcg respectively, ceftazaidime (30mcg), aztreonam (30mcg), ceftriaxone (30mcg) and cefotaxime (30mcg).
All isolates were screened for ESBL
production using four indicator Cephalosporins, namely, Cefotaxime (30 [micro]g), Cefpodoxime (10 [micro]g), Ceftazidime (30 [micro]g), and one monobactam that is Aztreonam (30 [micro]g).
In this study, 137 infections caused by enterobacteria with an ESBL
profile were identified, with a similar distribution between adult and pediatric services.
Distribution of 105 multilocus sequence types (MLSTs) among predominant extended-spectrum [beta]-lactamase (ESBL
) and carbapenemase gene types encoded by 196 ESBL-producing Escherichia coli from humans and food, Cambodia, 2015-2016.
In the developing nations the emergence of ESBL
producing pathogens is high and causing serious issues worldwide (KMendelsonG et al.,2005; Kumar et al., 2006; Bazzaz et al., 2009; ronenberg et al., 2013).
production was performed according to the Clinical and Laboratory Standards Institute (CLSI) guidelines of 2012.
Commercially available MBL-ESBL-AmpC Coexistence Detection MIC strips are superior compared to other phenotypic detection methods because of their composition (Clavulanic acid--a ESBL
inhibitor, Cloxacillin--a AmpC inhibitor, EDTA - MBL inhibitor).
There was a significant association between production of ESBLs
and higher antibiotic resistance in the tested isolates [Table 2].
Briefly, the test isolate was seeded on Mueller Hinton agar (MHA) as for ESBL
detection and two 30 pg cefoxitin disks (Abtek Biologicals Ltd, Liverpool, UK) were placed on the surface of the seeded MHA plate.
Evolution and epidemiology of extended-spectrum beta-lactamases (ESBLs
) and ESBL-producing microorganisms.
Antimicrobial Susceptibility Profiles and ESBL