Eagle's medium

Eagle's medium

[′ē·gəlz ‚mēd·ē·əm]
(microbiology)
A tissue-culture medium, developed by H. Eagle, containing vitamins, amino acids, inorganic salts and serous enrichments, and dextrose.
References in periodicals archive ?
Dulbecco's modified Eagle's medium (90%) and fetal bovine serum (10%) was the media of choice per standard lab protocols.
Briefly, we added the aliquots of the HPLC fractions (1-5 [micro]L) or HPLC-purified synthetic LTX-diol isomers in redistilled ethanol to cultured MCF-7 cells grown in phenol red-free Dulbecco's modified Eagle's medium (DMEM) containing 5% charcoal-stripped, sulfatase-treated fetal calf serum.
48 hours after infection, cells expressing drug resistance were selected by applying 500 -g/ml of G418 and/or 150 -g/ml of hygromycin B to 10% Fetal Bovine Serum (FBS) Dulbecco's modified Eagle's medium (DMEM).
Punches (3 mm) were obtained from the posterior sclera of chick eyes and immediately placed in multiwell plates containing Dulbecco's Modified Eagle's Medium (DMEM) alone (controls) or DMEM containing Tissue Inhibitor of Metalloproteinase CRIMP)--2, a natural inhibitor of gelatinase A, Nobiletin, a citrus flavonoid, or Captopril, an inhibitor of angiotensin converting enzyme, which also blocks activity of [Zn.
For isolation and differentiation of monocytes, PBMCs were suspended in mononuclear leukocyte culture medium [complete Dulbecco's modified Eagle's medium (DMEM)] (Waldman et al.
Dulbecco's modified Eagle's medium (DMEM), RPMI-1640, fetal calf serum (FCS), HEPES, L-glutamine, 2-mercaptoethanol, penicillin/streptomycin, bovine serum albumin (BSA), dinitrophenyl conjugated human serum albumin (DNP-HSA), p-nitrophenyl-N-acetyl-[beta]-D-glucopyranoside, and neutral red were obtained from Sigma Chemical Company (St.
NIH3T3 and MCF-7 cells were maintained in Dulbecco's Modified Eagle's Medium (DMEM) (Sigma) plus 5% calf serum (Gibco/Invitrogen Corp.
Briefly, the serum-free growth medium was composed of a 1:1 mixture of Dulbecco's modified Eagle's medium (DMEM) and Ham's F-12 supplemented with selenium (5 ng/mL), insulin (5 [micro]g/mL), transferrin (5 [micro]g/mL), hydrocortisone (36 ng/mL), triiodothyronine (4 pg/mL), and epidermal growth factor (10 ng/mD.
1] cells were grown in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, penicillin (100 U/mL), and streptomycin (100 [micro]g/mL) in a humidified atmosphere of 95% air and 5% C[O.
C6 cells (American Type Culture Collection, CCL 107) were maintained in Dulbecco's Modified Eagle's Medium supplemented with 5% inactivated fetal bovine serum and 100 [micro]g/mL penicillin--streptomycin.
We demonstrated that the UROtsa cells could be successfully placed into a serum-free growth medium consisting of a 1:1 mixture of Dulbeco's modified Eagle's medium and Ham's F-12 supplemented with selenium (5 ng/mL), insulin (5 [micro]g/mL), transferrin (5 [micro]g/mL), hydrocortisone (36 ng/mL), triiodothyronine (4 pg/mL), and epidermal growth factor (10 ng/mL).
We cultivated the cells in ZFL medium consisting of Liebowitz L-15, Dulbecco's Modified Eagle's Medium (DMEM), and Ham's F 12 (50:35:15) supplemented with 10 mg/mL insulin, 5% fetal bovine serum (Hyclone, Salt Lake City, UT), and 0.