Dulbecco's modified
Eagle's medium (DMEM), fetal bovine serum (FBS), and antibiotics (penicillin and streptomycin sulfate) were purchased from Life Technologies Inc.
Cells were seeded at 2.0 x [10.sup.5] cells/well in a 6-well plate containing 2 mL Dulbecco's modified
Eagle's medium plus 10% fetal bovine serum and 1% penicillin/streptomycin.
To serve as negative controls, 3 animals were inoculated intraperitoneally with Dulbecco's Modified
Eagle's Medium. Animals were monitored for signs of clinical illness, and body weight and temperature readings were obtained daily.
Dulbecco's modified
Eagle's medium (90%) and fetal bovine serum (10%) was the media of choice per standard lab protocols.
MDCK cell monolayers were incubated for 24 h with a mixture of conditioned medium and Dulbecco's modified
Eagle's medium (Gibco BRL) in equal proportions.
The buffy coat was taken in the test tube along with the media- Dulbecco's Modified
Eagle's Medium (DMEM) containing antibiotic and subjected for centrifugation for 10 minutes.
To test invasiveness of the strains Hep-2 cells were grown in minimal
Eagle's medium (MEM) supplemented with 10% fetal bovine serum (FBS), 2mM L-glutamine and 20 mM HEPES (Hi Media).
rotundifolia : Cell culture-Estrogen receptor-positive human breast adenocarcinoma MCF-7 cells provided by the Institute of Bio chemistry and Cell Biology were cultured in Dulbecco's modified
Eagle's medium (DMEM) containing antibiotics (100 IU/ml penicillin and 100 ug/ml streptomycin), supplemented with 10 per cent foetal bovine serum.
Briefly, we added the aliquots of the HPLC fractions (1-5 [micro]L) or HPLC-purified synthetic LTX-diol isomers in redistilled ethanol to cultured MCF-7 cells grown in phenol red-free Dulbecco's modified
Eagle's medium (DMEM) containing 5% charcoal-stripped, sulfatase-treated fetal calf serum.
48 hours after infection, cells expressing drug resistance were selected by applying 500 -g/ml of G418 and/or 150 -g/ml of hygromycin B to 10% Fetal Bovine Serum (FBS) Dulbecco's modified
Eagle's medium (DMEM).
Punches (3 mm) were obtained from the posterior sclera of chick eyes and immediately placed in multiwell plates containing Dulbecco's Modified
Eagle's Medium (DMEM) alone (controls) or DMEM containing Tissue Inhibitor of Metalloproteinase CRIMP)--2, a natural inhibitor of gelatinase A, Nobiletin, a citrus flavonoid, or Captopril, an inhibitor of angiotensin converting enzyme, which also blocks activity of [Zn.sup.2+]-dependent metalloproteinases.
For isolation and differentiation of monocytes, PBMCs were suspended in mononuclear leukocyte culture medium [complete Dulbecco's modified
Eagle's medium (DMEM)] (Waldman et al.