Eagle's medium

Eagle's medium

[′ē·gəlz ‚mēd·ē·əm]
(microbiology)
A tissue-culture medium, developed by H. Eagle, containing vitamins, amino acids, inorganic salts and serous enrichments, and dextrose.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
References in periodicals archive ?
Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and antibiotics (penicillin and streptomycin sulfate) were purchased from Life Technologies Inc.
Cells were seeded at 2.0 x [10.sup.5] cells/well in a 6-well plate containing 2 mL Dulbecco's modified Eagle's medium plus 10% fetal bovine serum and 1% penicillin/streptomycin.
To serve as negative controls, 3 animals were inoculated intraperitoneally with Dulbecco's Modified Eagle's Medium. Animals were monitored for signs of clinical illness, and body weight and temperature readings were obtained daily.
Dulbecco's modified Eagle's medium (90%) and fetal bovine serum (10%) was the media of choice per standard lab protocols.
MDCK cell monolayers were incubated for 24 h with a mixture of conditioned medium and Dulbecco's modified Eagle's medium (Gibco BRL) in equal proportions.
The buffy coat was taken in the test tube along with the media- Dulbecco's Modified Eagle's Medium (DMEM) containing antibiotic and subjected for centrifugation for 10 minutes.
To test invasiveness of the strains Hep-2 cells were grown in minimal Eagle's medium (MEM) supplemented with 10% fetal bovine serum (FBS), 2mM L-glutamine and 20 mM HEPES (Hi Media).
rotundifolia : Cell culture-Estrogen receptor-positive human breast adenocarcinoma MCF-7 cells provided by the Institute of Bio chemistry and Cell Biology were cultured in Dulbecco's modified Eagle's medium (DMEM) containing antibiotics (100 IU/ml penicillin and 100 ug/ml streptomycin), supplemented with 10 per cent foetal bovine serum.
Briefly, we added the aliquots of the HPLC fractions (1-5 [micro]L) or HPLC-purified synthetic LTX-diol isomers in redistilled ethanol to cultured MCF-7 cells grown in phenol red-free Dulbecco's modified Eagle's medium (DMEM) containing 5% charcoal-stripped, sulfatase-treated fetal calf serum.
48 hours after infection, cells expressing drug resistance were selected by applying 500 -g/ml of G418 and/or 150 -g/ml of hygromycin B to 10% Fetal Bovine Serum (FBS) Dulbecco's modified Eagle's medium (DMEM).
Punches (3 mm) were obtained from the posterior sclera of chick eyes and immediately placed in multiwell plates containing Dulbecco's Modified Eagle's Medium (DMEM) alone (controls) or DMEM containing Tissue Inhibitor of Metalloproteinase CRIMP)--2, a natural inhibitor of gelatinase A, Nobiletin, a citrus flavonoid, or Captopril, an inhibitor of angiotensin converting enzyme, which also blocks activity of [Zn.sup.2+]-dependent metalloproteinases.
For isolation and differentiation of monocytes, PBMCs were suspended in mononuclear leukocyte culture medium [complete Dulbecco's modified Eagle's medium (DMEM)] (Waldman et al.