Eagle's medium

[′ē·gəlz ‚mēd·ē·əm]

(microbiology)

A tissue-culture medium, developed by H. Eagle, containing vitamins, amino acids, inorganic salts and serous enrichments, and dextrose.

References in periodicals archive ?

Dulbecco's modified Eagle's medium (90%) and fetal bovine serum (10%) was the media of choice per standard lab protocols.

Assessing the morphology of human gingival fibroblasts upon exposure to dental adhesives in the presence of porphyromonas gingivalis lipopolysaccharide

Briefly, we added the aliquots of the HPLC fractions (1-5 [micro]L) or HPLC-purified synthetic LTX-diol isomers in redistilled ethanol to cultured MCF-7 cells grown in phenol red-free Dulbecco's modified Eagle's medium (DMEM) containing 5% charcoal-stripped, sulfatase-treated fetal calf serum.

Leukotoxin diols from ground corncob bedding disrupt estrous cyclicity in rats and stimulate MCF-7 breast cancer cell proliferation

48 hours after infection, cells expressing drug resistance were selected by applying 500 -g/ml of G418 and/or 150 -g/ml of hygromycin B to 10% Fetal Bovine Serum (FBS) Dulbecco's modified Eagle's medium (DMEM).

Retroviral-mediated transfer and expression of the neomycin and hygromycin B drug resistance genes. (Abstract)

Punches (3 mm) were obtained from the posterior sclera of chick eyes and immediately placed in multiwell plates containing Dulbecco's Modified Eagle's Medium (DMEM) alone (controls) or DMEM containing Tissue Inhibitor of Metalloproteinase CRIMP)--2, a natural inhibitor of gelatinase A, Nobiletin, a citrus flavonoid, or Captopril, an inhibitor of angiotensin converting enzyme, which also blocks activity of [Zn.

Inhibition of scleral gelatinase a activity by captopril. (Collegiate Communications--Undergraduate)

For isolation and differentiation of monocytes, PBMCs were suspended in mononuclear leukocyte culture medium [complete Dulbecco's modified Eagle's medium (DMEM)] (Waldman et al.

Comparison of ultrastructural cytotoxic effects of carbon and carbon/iron particulates on human monocyte-derived macrophages

Dulbecco's modified Eagle's medium (DMEM), RPMI-1640, fetal calf serum (FCS), HEPES, L-glutamine, 2-mercaptoethanol, penicillin/streptomycin, bovine serum albumin (BSA), dinitrophenyl conjugated human serum albumin (DNP-HSA), p-nitrophenyl-N-acetyl-[beta]-D-glucopyranoside, and neutral red were obtained from Sigma Chemical Company (St.

Environmentally relevant metal and transition metal ions enhance Fc[epsilon]RI-mediated mast cell activation. (Research)

NIH3T3 and MCF-7 cells were maintained in Dulbecco's Modified Eagle's Medium (DMEM) (Sigma) plus 5% calf serum (Gibco/Invitrogen Corp.

Antiandrogenic activity and metabolism of the organophosphorus pesticide fenthion and related compounds. (Research)

Briefly, the serum-free growth medium was composed of a 1:1 mixture of Dulbecco's modified Eagle's medium (DMEM) and Ham's F-12 supplemented with selenium (5 ng/mL), insulin (5 [micro]g/mL), transferrin (5 [micro]g/mL), hydrocortisone (36 ng/mL), triiodothyronine (4 pg/mL), and epidermal growth factor (10 ng/mD.

Expression of hsp 27, hsp 60, hsc 70, and hsp 70 stress response genes in cultured human urothelial cells (UROtsa) exposed to lethal and sublethal concentrations of sodium arsenite

1] cells were grown in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, penicillin (100 U/mL), and streptomycin (100 [micro]g/mL) in a humidified atmosphere of 95% air and 5% C[O.

Attenuation of both apoptotic and necrotic actions of cadmium by Bcl-2. (Articles)

C6 cells (American Type Culture Collection, CCL 107) were maintained in Dulbecco's Modified Eagle's Medium supplemented with 5% inactivated fetal bovine serum and 100 [micro]g/mL penicillin--streptomycin.

Developmental neurotoxicity of chlorpyrifos modeled in vitro: comparative effects of metabolites and other cholinesterase inhibitors on DNA synthesis in PC12 and C6 cells. (Articles)

We demonstrated that the UROtsa cells could be successfully placed into a serum-free growth medium consisting of a 1:1 mixture of Dulbeco's modified Eagle's medium and Ham's F-12 supplemented with selenium (5 ng/mL), insulin (5 [micro]g/mL), transferrin (5 [micro]g/mL), hydrocortisone (36 ng/mL), triiodothyronine (4 pg/mL), and epidermal growth factor (10 ng/mL).

The immortalized UROtsa cell line as a potential cell culture model of human urothelium

We cultivated the cells in ZFL medium consisting of Liebowitz L-15, Dulbecco's Modified Eagle's Medium (DMEM), and Ham's F 12 (50:35:15) supplemented with 10 mg/mL insulin, 5% fetal bovine serum (Hyclone, Salt Lake City, UT), and 0.

Green fluorescent protein (GFP) as a marker of aryl hydrocarbon receptor (AhR) function in developing zebrafish (danio rerio). (Articles)

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