Various methods such as differential plating, velocity sedimentation, elutriation
, discontinuous gradient, Hoechst 33342 and rhodamine123 side population, magnetic-activated cells sorting (MACS) and fluorescence activated cells sorting (FACS) have been employed to isolate SSC in different species (van Pelt et al.
The influence of the particle size distribution in the bed on the elutriation
rate of fine particles was negligible.
Human monocytes were obtained from 3 healthy donors by leukapheresis and counterflow elutriation
Thieulant ML, Duval J (1985) Differential distribution of androgen and estrogen receptors in rat pituitary cell population separated by centrifugal elutriation
Subsamples (100 g) of the surface soil collected for aggregate assessment were placed in a hydropneumatic elutriation
chamber which blasted the soil with pressurised water and washed the dispersed soil through a 0.
Handle up to 10,000 lb/hr and use various methods of washing and separation such as sorting, elutriation
, agitation, and hydrocyclones.
The procedure was monitored at all times and a portion of the impregnated resin was taken and the precious metals were extracted partially by elutriation
and partially by ashing.
In this study, we also applied the technique of centrifugal elutriation
, however, the large hyalinocytes and small hyalinocytes could not be separated in this study.
Monocyte were isolated on a large scale from EDTA whole blood (450 mL) from blood donors by density gradient centrifugation, purified by elutriation
to a purity of >90%, and cryopreserved in RPMI 1640 (Gibco) containing 250 mL/L acid-treated fetal calf serum (Flow Laboratories) or 100 mL/L dimethyl sulfoxide (article no.
Custom-designed systems handle up to 10,000 lb/hr and use various methods of washing and separation such as sorting, elutriation
, agitation, float/sink, and hydrocyclones.