endonuclease

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endonuclease

[¦en·dō′nü·klē‚ās]
(biochemistry)
Any of a group of enzymes which degrade deoxyribonucleic acid or ribonucleic acid molecules by attaching nucleotide linkages within the polynucleotide chain.
References in periodicals archive ?
An exonucleolytic activity of human apurinic/apyrimidinic endonuclease on 3' mispaired DNA.
It is likely that many of the 12 restriction endonucleases utilized did not yield differing haplotypes because these areas of the amplicon are highly conserved.
Therefore, the results from two different endonucleases were combined.
Conventionally, the use of methylation-sensitive restriction endonucleases involves the digestion of unmethylated CpG sites in a DNA sequence, so as to avoid selective PCR amplification of the uncut DNA sequence containing methylated CpG sites.
According to research report " Molecular Biology Enzymes, Kits, & Reagents Market by Application (Cloning, Epigenetics, PCR, Restriction Digestion, Sequencing), Product (Ligase, Phosphatase, Polymerase, Protease, Restriction Endonuclease, Reverse Transcriptase) - Global Forecast to 2018 " published by MarketsandMarkets, report provides a detailed overview of the major drivers, restraints, challenges, opportunities, current market trends, and strategies impacting the global molecular biology enzymes, kits & reagents market along with the estimates and forecasts of the revenue and market share analysis.
php) for identifying endonucleases applicable for the genotyping of the 2 SNVs.
The resulting double digested solution was extracted with phenol:chloroform to remove the restriction endonucleases, and the DNA was concentrated by ethanol precipitation.
The protocol for DNA sequencing by indexer walking consists of 3 consecutive steps: (a) DNA is amplified by PCR, followed by DNA sequencing with the universal primer (M13/pUC forward or reverse primer, or the common indexing primer; Table 1); (b) the obtained nucleotide sequence is then analyzed with a computer program to find specific sites recognized by type IIS restriction endonucleases located close to its 3' end and to determine the composition of the 4-nt protruding ends produced by each enzyme; and (c) analyzed DNA is partially digested with a selected type IIS restriction endonuclease.
The A24R gene was used as marker for the nonvariable region of the virus genome, and the PCR amplicons were digested with the endonucleases SspI and RsaI (New England Biolabs, Beverly, MA, USA), as recommended by the manufacturer.
DNA damage-specific endonucleases from Micrococcus luteus have been used previously in combination with sucrose gradient sedimentation (8), alkaline elution (9), and alkaline unwinding (10) to reveal base damage inflicted by ionizing radiation.
The repository of knowledge, generated during the almost four decades of restriction endonuclease study at NEB, has fueled a discovery program that has radically expanded the utility of restriction endonucleases and activity on a variety of DNA templates, including those created by epigenetic modification.