endonuclease

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endonuclease

[¦en·dō′nü·klē‚ās]
(biochemistry)
Any of a group of enzymes which degrade deoxyribonucleic acid or ribonucleic acid molecules by attaching nucleotide linkages within the polynucleotide chain.
References in periodicals archive ?
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Digestion spectrum of four selected restriction endonucleases applied to 10 novel Chinese isolates and FV3 (the type species) to determine conflicts with the "standard spectrum" suggested by OIE.
It took years, but in 2011, Burt and colleagues announced in Nature that they had created a homing endonuclease that could find and cut a gene in mosquitoes.
Cloning kit (Inset/Aclone PCR product cloning kit), DNA extraction kit from Agarose gel kit, RT PCR kit and restriction endonuclease were obtained from Fermentase (Switzerland).
Mutagenic scan of the H-N-H motif of colicin E9: implications for the mechanistic enzymology of colicins, homing enzymes and apoptotic endonucleases.
Generally, to produce sticky or complementary ends for sticky-end DNA cloning, insert DNA and vector are separately cut with same restriction endonuclease enzymes.
Further RFLP analyses using additional endonucleases are needed for a better understanding of the significance of this molecular variation.
marker 2-Overall quality of the Agarose gel electrophoresis and pDNA estimation of the ccc form and matching of the size with depository data 3-Control of the Digestion of the pDNA with a single Restriction profile endonuclease cutter and estimation of the band size Double digestion with two endonucleases that release the inserted DNA sequence of interest [cDNA] and size estimation.
To determine the optimal concentration of magnesium, digestions were carried out by using 1 [micro]g of recombinant enzyme, 1 [micro]g of covalently closed circular DNA and a buffer suitable for endonucleases containing: 10 mM Tris x Cl at pH 7.
After the digestion of DNA with methylation-sensitive endonucleases, the proportion of uncut (and thus methylated) DNA molecules is quantified by qPCR, with undigested DNA used as a reference.
Also our previous study showed that apoptosis caused by I/R injury may be due to action endonuclease or by acting on cell organelles, alternating signal transduction pathways or affecting the intracellular enzymes responsible for proper functioning and survival of the cell [11, 12].
Potentially useful restriction endonucleases identified by the virtual digestion analyses were then tested in a PCR/ RFLP agarose gel analysis of the ITS-1 marker in multiple individuals of each species.