Endopeptidase


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Related to Endopeptidase: neutral endopeptidase

endopeptidase

[¦en·dō′pep·tə‚dās]
(biochemistry)
An enzyme that acts upon the centrally located peptide bonds of a protein molecule.

Endopeptidase

 

an enzyme of the class of hydrolases. Endo-peptidases catalyze the decomposition of internal peptide bonds in proteins and peptides. The speed of hydrolysis of a given peptide bond by different endopeptidases depends on the substrate specificity of the enzyme and the spatial accessibility of the given peptide bond, especially if the substrate is a native, not denatured, protein. It does not depend on the size of the molecules of the substrate, that is, the length of the polypeptide chain. The substrate specificity of an endopeptidase consists in the ability of a given enzyme to hydrolyze the peptide bonds between certain amino acid residues with the greatest speed. For example, chy-motrypsin very rapidly hydrolyzes peptide bonds together with aromatic amino acids; trypsin does the same with diamino acids. Endopeptidases are classified as extracellular or intracellular (tissular). Extracellular endopeptidases are secreted into the digestive cavity of animals (for example, enterokinase) or simply into the external environment (the exo-enzymes of bacteria).

The use of endopeptidases in determining the primary structure of proteins is based on their properties.

References in periodicals archive ?
"After validation with respect to the LD50 assay (reference method), the product may also be assayed by other methods that are preferable in terms of animal welfare, for example mouse bioassays using paralysis as the end-point, ex vivo assays using mouse phrenic nerve diaphragm, endopeptidase assays in vitro and cell-based assays.
The treatment of nine immunogenic epitopes of the 26-mer and 33-mer gliadin fragments by prolyl endopeptidase from Aspergillus niger (AN-PEP) successfully degraded all the nine epitopes in the stomach pH range at considerable lesser dosage than the digestive enzyme supplements.
Proteinases or endopeptidases are protease enzymes that hydrolyze internal peptide bonds in a protein.
ACE2 encodes angiotensin-converting enzyme-2, MME encodes neprilysin (neutral endopeptidase), PRCP encodes prolylcarboxypeptidase, PREP encodes prolylendopeptidase, and MAS1 encodes MAS (Ang 1-7 receptor).
Natriuretic peptide receptor type C and neutral endopeptidases actively clear BNP from the circulation in addition to renal clearance; the plasma half-life is thus short, approximately 20 minutes.
Roques, "Autoradiographic comparison of the distribution of the neutral endopeptidase "enkephalinase" and of mu and delta opioid receptors in rat brain," Proceedings of the National Academy of Sciences of the United States of America, vol.
Truncating mutations in the metallomembrane endopeptidase (MME) gene were found to be causative of alloimmunization during pregnancy, leading to the production of anti-NEP antibodies.
Since these enzyme lack poly endopeptidase activity, relatively large peptide fragment accumulate in small intestine32.
Global Markets Direct's, 'Neprilysin (Neutral Endopeptidase 24.11 or Atriopeptidase or Common Acute Lymphocytic Leukemia Antigen or CD10 or EC 3.4.24.11) - Pipeline Review, H1 2016', provides in depth analysis on Neprilysin (Neutral Endopeptidase 24.11 or Atriopeptidase or Common Acute Lymphocytic Leukemia Antigen or CD10 or EC 3.4.24.11) targeted pipeline therapeutics.
(Lepidoptera: Bombycidae), secretions of the glandula prostatica, which contain an endopeptidase called initiatorin, trigger a cascade of reactions in the apyrene sperm (Osanai et al.
[4.] Rudakova NL, Balaban NP, Danilova YV, Rudenskaya GN, Sharipova MR (2010) Characteristics of a novel secreted zinc-dependent endopeptidase of Bacillus intermedius.