Enrichment Culture


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enrichment culture

[in′rich·mənt ‚kəl·chər]
(microbiology)
A medium of known composition and specific conditions of incubation which favors the growth of a particular type or species of bacterium.

Enrichment Culture

 

a culture containing microorganism cells grown on a selective nutrient medium. Enrichment cultures were proposed by the Russian microbiologist S. N. Vinogradskii. The special composition of the nutrient media creates conditions that promote the growth of microorganisms with certain physiological properties. For example, when soil, water, or sediment from a body of water is cultured on a medium containing glucose and several mineral salts but no nitrogen compounds, nitrogen-fixing microorganisms begin to grow in the culture. Enrichment cultures of bacteria that decompose cellulose can be obtained on a medium containing cellulose as the sole source of carbon.

Enrichment cultures must always be prepared before pure cultures of the microorganisms can be isolated. They can be obtained in the presence of growth factors, such as vitamins and amino acids, by adding a smaller number of bacterial cells to the medium. As a result, four to ten times more microorganisms can be found in soil and water than when they are cultured on media lacking growth factors.

A. A. IMSHENETSKII

References in periodicals archive ?
Table 1: Days that were taken to break the filter paper strips down by every generation of enrichment cultures from different sources during the continuously transferring over 8 months
Among the 103 meat enrichment cultures, 90 were positive for eae gene and one among them was positive for all the STEC genes.
However, PCR on enrichment cultures gave better results.
Metabolic by products of anaerobic toluene degradation by sulfate-reducing enrichment cultures.
Degradation of toluene and m-xylene and transformation of o-xylene by denitrifying enrichment cultures.
A diagnosis of botulism was established 19 days after onset of symptoms by detection of toxin type B in stool enrichment cultures.
Sahin and Tamer (2000) isolated a number of species of bacteria and fungi from enrichment cultures containing the fungicide Thiram.
PCR of DNA isolated from methanotrophic enrichment cultures were performed with primers specific to 16S rRNA genes of type I and type II MTs.
The kits provide rapid detection of pathogen DNA isolated from enrichment cultures of potentially contaminated food samples in addition to specific Internal Controls.