Enrichment Culture


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enrichment culture

[in′rich·mənt ‚kəl·chər]
(microbiology)
A medium of known composition and specific conditions of incubation which favors the growth of a particular type or species of bacterium.

Enrichment Culture

 

a culture containing microorganism cells grown on a selective nutrient medium. Enrichment cultures were proposed by the Russian microbiologist S. N. Vinogradskii. The special composition of the nutrient media creates conditions that promote the growth of microorganisms with certain physiological properties. For example, when soil, water, or sediment from a body of water is cultured on a medium containing glucose and several mineral salts but no nitrogen compounds, nitrogen-fixing microorganisms begin to grow in the culture. Enrichment cultures of bacteria that decompose cellulose can be obtained on a medium containing cellulose as the sole source of carbon.

Enrichment cultures must always be prepared before pure cultures of the microorganisms can be isolated. They can be obtained in the presence of growth factors, such as vitamins and amino acids, by adding a smaller number of bacterial cells to the medium. As a result, four to ten times more microorganisms can be found in soil and water than when they are cultured on media lacking growth factors.

A. A. IMSHENETSKII

References in periodicals archive ?
Sulfate reducing enrichment cultures obtained from the two sites showing TCE degradation to cis-DCE, 42 and 44, as well as the two sites showing nearly complete degradation of TCE to VC, 48 and 50.
Anaerobic oxidation of n-dodecane by an addition reaction in a sulfate-reducing bacterial enrichment culture.
Procedures for preparing foods for isolation of Salmonella by enrichment culture generally recommend that a 25-g analytical unit be diluted in a 1:9 sample:broth ratio, for example, 25 g (ml) of sample plus 225 ml of pre-enrichment broth.
In the test, a sample of enrichment culture is placed into the sample port.
The beads have a magnetite core and a ceramic zirconium oxide coating and are designed for the immunomagnetic separation of target bacteria from enrichment cultures.