Enzyme Preparation

Enzyme Preparation

 

an enzyme-containing drug that influences metabolism.

Enzyme preparations are obtained from plants, microorganisms and products of animal origin. Gastric juice, pepsin, and pancreatin are used to treat gastrointestinal diseases involving dysfunction of the digestive glands. Proteolytic enzyme preparations obtained from bovine pancreas, for example, chymotrypsin, are widely used in medical practice; they split the peptide bonds in proteins and peptides. When used locally, trypsin destroys necrotic tissue and fibrinous masses and liquefies viscous secretions, exudates, and blood clots. Intramuscular injections of trypsin have an anti-inflammatory effect. Trypsin preparations are inhaled or are injected intramuscularly to eliminate secretions and exudates in bronchitis and bronchiectasis and to treat thrombophlebitis, osteomyelitis, maxillary sinusitis, and iridocyclitis. Trypsin preparations are also applied locally to burns, decubitus ulcers, and purulent wounds.

The enzyme deoxyribonuclease liquefies pus and inhibits the development of herpesvirus and adenovirus; it is consequently used to treat herpetic and adenoviral eye infections, abscesses of the lungs, and lesions of the upper respiratory tract. Preparations containing the enzyme hyaluronidase increase tissue permeability and facilitate the flow of fluids in interstitial areas; they are used in the treatment of joint contractures, scars remaining after burns and surgery, and hematomas. Thromboembolism, thrombophlebitis, and myocardial infarction are treated with fibrinolysin, which dissolves newly formed thrombi. Penicillinase obtained from a Bacillus cereus culture inactivates penicillin drugs and is used to control allergic reactions caused by these drugs.

Enzyme inhibitors are also used in medical practice. These include anticholinesterase agents that inhibit cholinesterase, and certain antidepressants that inhibit monoamine oxidase. Carbonic anhydrase inhibitors such as Diacarb are used as diuretics, and such inhibitors of proteolytic enzymes as Trasylol are used to treat acute pancreatitis.

REFERENCES

Kaplanskii, S. Ia. “Primenenie fermentnykh preparatov v terapii razlichnykh zabolevanii.” In Aktual’nye voprosy sovremennoi biokhimii, vol. 2. Moscow, 1962.
Mashkovskii, M. D. Lekarstvennye sredstva, 7th ed., part 2. Moscow, 1972.

V. V. CHURIUKANOV

References in periodicals archive ?
Materials: AOG (Fungal Lactase) which have 7.7 IU/g activity, is a commercial enzyme preparation, was provided as a gift by Bio-Cat (Troy, USA).
The reaction mixture containing 0.25 ml enzyme preparation and 0.25 ml methyl gallate as a substrate (0.01M methyl gallate prepared in 0.05 M citrate buffer, pH 5.0).
The enzyme preparation was tested for contaminating levels of other enzymes using the method of dinitroalicylic acid [9].
Approved in 2010, PANCREAZE is a pancreatic enzyme preparation consisting of pancrelipase, an extract derived from porcine pancreatic glands, as well as other enzyme classes, including porcine-derived lipases, proteases and amylases.
Lumbrokinase is a complex enzyme preparation extracted from earthworms.
The biochemical properties of recombinant CtCel6 made it potentially effective for bioconversion of biomass and had tremendous potential in industrial applications such as enzyme preparation industry and feed processing industry.
Krueger NA, Adesogan AT, Staples CR, Krueger WK, Dean BD, Littell RC (2008) The potential to increase digestibility of tropical grasses with a fungal, ferulic acid esterase enzyme preparation. Anim.
(2004) who studied the effect of endofeed DC (enzyme preparation containing endo 1-3 AY glucanase and endo1-4 AY xylanase) in cereal base diet containing enzyme@ 0 and 125mg/kg and reported that enzyme supplementation have significantly improved effect on FCR/kg egg mass.
However, purified lecithin isolated from the same crude lipids was not hydrolyzed with the enzyme preparation. He thought that there was an additional factor in the crude liver lipids which was responsible for the P.
For pH stability assay, the enzyme preparation was diluted (1:2) in McIlvaine buffer in a pH range from 3.0 to 7.0 and incubated at 4[degrees]C for 24h.
9.0) was mixed with 50 mL of crude enzyme preparation. Saccharification was performed in shake flasks (120 rpm) at 37[degrees]C for 24 and 48 hours.
The culture broth containing phytase was stored at 4[degrees]C and used as crude enzyme preparation for further studies.

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