However, in the experiment I, there was also a significant effect for the explant type, with emphasis on the epicotyl
; and in experiment II, there was interaction between the factors.
consists of a uniseriate and hairy epidermis, and collenchymatous and parenchymatous cortex, without typical endodermis.
Hypocotyl and epicotyl
explants (2 cm in length) were transferred to WPM supplemented with 6-benzyladenine (BA) (0, 2.5, 5.0, and 10.0 [micro]M) alone or combined with indole-3-butyric acid (IBA) or [alpha]-naphthaleneacetic acid (NAA) (0.5 [micro]M).
Double coty-node explants were obtained by excising out the epicotyls
at the cotyledonary junctions and cutting off the hypocotyls 45 mm beneath the cotyledons.
Shoot regeneration of lentil has been previously reported from meristem tips, shoot tips and shoot meristems (Bajaj and Dhanju, 1979; Williams and McHughen, 1986; Singh and Raghuvanshi, 1989; Polanco and Ruiz, 1997), epicotyl
(Williams and McHughen, 1986), embryonic axes (Saxena and King, 1987), first nodes and bractlets of immature seeds (Polanco et al., 1988), cotyledonary seedlings (Mallick and Rashid, 1989), nodal segments (Singh and Raghuvanshi, 1989), stem and cotyledonary nodes (Warkentin and McHughen, 1993; Polanco, 2001; Khawar and Ozcan 2002a, Sarker et al., 2003; Khawar et al., 2004; Sevimay et al., 2005) and decapitated embryo (Sarker et al., 2003).
In hypogeal emergence, the cotyledons remain beneath the soil surface and the epicotyl
emerges from the soil.
The growing point of the shoot above the point of cotyledon attachment is called the epicotyl
, and the section of stem below the cotyledons is called the hypocotyl.
This exercise is introduced with a description of seedling morphology, including root hairs, cotyledons, hypocotyl, epicotyl
Soybean BRU1 encodes a functional xyloglucan endotransglycosylase that is highly expressed in inner epicotyl
tissues during brassinosteroid-promoted elongation.
The Ministry of Agriculture and the GCNA are also hard at work developing plants from mature female trees using epicotyl
grafting, a technique that produces short bushy plants with strong root systems and the capacity to bear fruit within three to four years.
emerges and the cotyledons remain below the soil surface (for example, corn) (Figure 6-23b).