It is intended for single and extremely long-term acquisition of a large amount of image data from live and fixed samples using transmission and epifluorescence microscopy
While most previous studies used epifluorescence microscopy
to observe spindles two-dimensionally, we employed 3D observation using confocal fluorescence microscopy in order to determine the size and shape of spindles more accurately.
The investigators used atomic force microscopy and epifluorescence microscopy
to assess the spatial distribution of bacteria as they dried from droplets.
In standard epifluorescence microscopy
, used in most laboratories for immunofluorescence of renal and skin biopsies, or fluorescence in situ hybridization of individual chromosome probes, no mechanism exists to restrict out-of-focus light from being imaged.
6] oocysts/g feces was determined by using epifluorescence microscopy
at 400x magnification.
The direct method used epifluorescence microscopy
, with samples taken directly from the treated and untreated tanks, filtered and immediately counted under the microscope and expressed as colony forming units per mL (CFU/mL).
Whole brain was dissected and first examined directly without fixation using epifluorescence microscopy
(Olympus, Hamburg, Germany) to determine if GFP was being expressed.
Direct acridine ornge counts using epifluorescence microscopy
also showed a reduction in the number of cells on anodized alloy.
They borrowed an imaging technique called epifluorescence microscopy
from research on animal cells.
The nuclear status of non- fertilized oocytes was evaluated by epifluorescence microscopy
and the maturational stage was determined (1).
In situ denitrification gas samples were obtained using the acetylene block method; the DEA method determines the potential denitrifying capacity of samples under non-limiting conditions for denitrifying bacterial metabolism; bacterial biomass was determined using epifluorescence microscopy
coupled with computer assisted image analysis; and microbial community diversity was analyzed using the terminal restriction length polymorphism (T-RFLP) analysis.
Microbiological techniques such as epifluorescence microscopy
and image analysis are also taking their place.