In general, monoclonal antibody analysis will result in one epitope
, whereas polyclonal antibody analysis may result in numerous epitopes
MAbs 920, 441, 625 (for the epitopes
comprising amino acid residues 135-145, 148-158, and 169-178, respectively) were used as the control antibodies.
EP-1090 encodes 21 CTL epitopes
from conserved regions of multiple HIV virus proteins.
Identification of neutralizing and nonneutralizing epitopes
in the porcine reproductive and respiratory syndrome virus GP5 ectodomain.
These results demonstrate that protein structure plays an important role in the stability of this allergen to resist digestion and provide a link between food allergen structure, stability to digestion, and the immunodominant IgE-binding epitopes
within a population of food-allergic individuals.
The question is whether such an expression library immunization (ELI) vaccine could deliver multivalent epitopes
to drive immune responses to HIV.
Ninety-one percent of patients treated with EP-2101, who were tested for immune response, had a measurable response to at least one of the epitopes
included in the vaccine with 64% responding to at least three of the epitopes
Samples from the rest of the patients, however, either reacted with the epitopes
in a broadly heterogeneous pattern (n = 8) or did not recognize any of the epitopes
in our panel of Tg-Mabs (n = 13; Fig.
The EP-2101 cancer vaccine candidate used in these trials includes nine CTL epitopes
from four tumor associated antigens (TAA) including two proprietary native epitopes
and seven modified, or analog, epitopes
announced its anticipated launch of Phase I human clinical trials with a therapeutic hepatitis B vaccine incorporating our epitope
were identified, and transgenic mice with the HLA-DQ locus exhibited strong T-cell responses to short ragweed extracts.
This problem may be circumvented by using polyclonal antibody preparations or MAb cocktails that bind multiple epitopes
in the targeted antigen.