The bioassays were carried out in 50 mL Erlenmeyer flask
by adding 20 ml of the sterile YM medium with adjusted pH and d-limonene (2%, v/v).
3-HPA production experiments were carried out as per the experimental design in 100-ml Erlenmeyer flasks
, containing 40 ml of production medium, supplemented with glucose (3.2, 3.6, 4.0), semicarbazide HCl (1.4, 1.8, 2.2), [K.sub.2]HP[O.sub.4,] (0.4, 0.7, 1.0) and pH was adjusted (6, 7, 8).
Therefore these isolates were screened for better enzyme activity using SSF technique in 250 ml Erlenmeyer flasks
containing 100 ml of M1 medium containing (in g/ L) NaN[O.sub.3], 2; KCl, 0.5; MgS[O.sub.4], 0.5; glucose, 10; Fe[Cl.sub.3], 10 mg; Ba[Cl.sub.2], 0.2; and Ca[Cl.sub.2]0.5; pH 6.8 and different concentration of pesticide was added.
H2SO4 (2 ml) in the Erlenmeyer flask
. The reaction mixture was refluxed at water bath (100 oC) for five hours.
In shaking flask batch fermentations were performed in 250ml Erlenmeyer flask
containing 100ml of corn cob hydrolysate medium by adding 10 g [l.sup.-1] yeast extract, 20 g [l.sup.-1] peptone, 0.5 g [l.sup.-1] [K.sub.2]HP[O.sub.4], 0.5 g [l.sup.-1] K[H.sub.2] P[O.sub.4], 2 g [l.sup.-1] [(N[H.sub.4]).sub.2] S[O.sub.4], 0.5 g [l.sup.-1] MgS[O.sub.4] x 7[H.sub.2]O and pH 6.0 and cultivated under aerobic condition on rotary-shaker at 200 rpm, 30[degrees]C for 96 hours.
was grown in a 250 ml Erlenmeyer flask
containing 50 ml medium GMS glucose mineral salts (yeast extract 3.0,Na2Po4 5.35, NH4Cl 2.67, Glucose 10.0, FeSO4.7H2O 0.4, MnSO4.7H2O 0.075 , mGso4.7H2O 0.1, CaCl2.2H2O 0.1 g/l, pH7.0, at 30[+ or -]1[degrees]C and 150 rpm, for 72 h).Cell suspension was used for inoculums (2%).
Equipment were used in this study includes heater equipped to magnetic mixer, autoclave, disposable 8 centimeter plate, flame light connected to gas, 10 centimeter glassy tube, erlenmeyer flask
. This study is tentative types of studies.
A.vinelandii was transferred from Burk's Nitrogen free agar medium plates to Burk's Nitrogen free medium (25 mL) contained in Erlenmeyer flask
at 80-90AdegC, shaked (5 c/s for 10 minutes) and filtered to obtain homogenized solution which was kept at 4AdegC in 250 mL Erlenmeyer flask
. In each and every step of the experiment, sterility conditions were maintained for the efficacy and accuracy in results without any impurity.
CSM (50 g) was transferred into a 500 mL Erlenmeyer flask
, inoculated with 10% (v/w) of A.
Subsequently, 5 ml of this solution was added with the ammonia buffer in an Erlenmeyer flask
to adjust the pH to 10.