We studied the methylation profiles of erythroblasts
and other blood cells (neutrophils, B-lymphocytes, and T-lymphocytes) and tissues (liver, lung, colon, small intestines, pancreas, adrenal gland, esophagus, heart, brain, and placenta) from the BLUEPRINT Project and the Roadmap Epigenomics Project and methylomes generated by our group (18-20, 23).
ERFE is a newly identified hormone, produced by erythroblasts
, following EPO stimulus, which blocks hepcidin production [6, 33, 34].
High expression of cell surface markers for erythroblasts
(glycophorin A), megakaryocytes (CD41), and granulocytes (MPO) was observed.
An EryD region was added according to the results by Chen et al., who localized a gate where orthochromic erythroblasts
with nuclear-cytoplasmic dyssynchrony fall .
Bone marrow cells consist of erythroblasts
, neutrophils, eosinophils, basophils, monoid cells (monocytes containing mesenchymal stem cells and macrophages), lymphocytes, and plasma cells .
CALR expression in erythroblasts
and granulocytes is also downregulated from [CD34.sup.+] cells, suggesting CALR's crucial role in platelet functions.
Radioprotective effects of the thiols GSH and WR-2721 against X-ray-induction of micronuclei in erythroblasts
. Mutat Res.
(That is, the assumption that fetal cells are available only by [uterine] invasive methods was not strictly correct.) These fetal cells include erythroblasts
, trophoblasts, granulocytes, and lymphocytes, all of which are nucleated and could theoretically be amenable to cytogenetic analysis.
In adults, HLA-G is found in the cornea, thymus, pancreas, endothelial cell precursors, and erythroblasts
The cord blood unit (CBU) contained 45.8 mL blood, cryopreserved by 6.0% DMSO (w/v), with a total nucleated cell count (TNC) of 2.53 x 10e8 mononuclear cells without erythroblasts
, but including 0.72 x 10e6 CD34+ (15.8/mL), and 0.23 x 10e6 colony forming cells (CFC) (vitality 84.4%, hemoglobin 91.8 [mg/mL]).
The biliary canaliculi had less microvillus in the experimental group and the erythroblasts
were atypically condensed, so that the heterochromatin appeared to be more abundant (Figs.
Together with muscle cells these differentiated cells are among the most radioresistant in the human body, while the most sensitive cells are spermatogonia and erythroblasts
, epidermal stem cells and gastrointestinal stem cells.