DRIHUIS (2000) previously showed that the addition of a silage inoculant containing Lactobacillus plantarum strains to high dry matter grass silage improved lactic fermentation and successfully prevented ethanol fermentation
. However, in the present study, LAB inoculation alone did not alter the fermentation to a more lactic pattern, despite Lactobacillus plantarum having high osmotolerance (WHITER & KUNG, 2001).
Then, 5% (v/v) yeast inoculum was added and cultured further (30[degrees]C, 180 rpm, 36 h) for ethanol fermentation
. The prehydrolysis step generated ~12.1 [+ or -] 0.7 g/L glucose in the flask for yeast cell growth without the lag phase.
Key words: Garcinia cambogia residue, Plackett-Burman design, Ethanol fermentation
, Hydroxycitric acid.
begins with the completion of glycolysis, which is also termed as EMP (Embden-Meyerhoff-Parnas) pathway (Dien et al., 2003).
Lin Y, Zhang W, Li C, Sakakibara K, Tanaka S, Kong H (2012) Factors affecting ethanol fermentation
using Saccharomyces cerevisiae BY4742.
Bacterial cellulose membrane--A new support carrier for yeast immobilization for ethanol fermentation
. Process Biochemistry, 46(10), 2054-2058.
plantarum control during the whole ethanol fermentation
(2009), obtaining quantitative data concerning the impact of inoculation size on yeast growth and metabolism is of great importance for industrial ethanol fermentation
The NREL researchers were also able to recover 82 per cent-87 per cent of the lipids from the CAP, even after ethanol fermentation
and distillation, indicating that the initial fermentation of sugars in the pretreated biomass slurry does not significantly impede lipid recovery.
Besides the five- and six-carbon sugars produced during hydrolysis of lignocellulosic biomass, several inhibitors of ethanol fermentation
are also generated.
Since fermentative microorganisms must be capable of surviving the high temperatures of SSF/SSCombF/CBP processes, further research is required: high temperature ethanol fermentation
is an emerging technology provided appropriate microorganisms can be developed.