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Nucleic Acid detection (using Ethidium
Bromide, SYBR Green Stain, SYBR Safe),2.
aureus, promoting extrusion of chemically unrelated compounds, such as ethidium
bromide, quaternary amine compounds, chloramphenicol and fluoroquinolones from the cell (Costa, Viveiros, Amaral, & Couto, 2013).
Following incubation with the reagents cells must be washed to reduce the possibility of background fluorescence from unspecific extracellular binding of ethidium
homodimer or hydrolysis in aqueous solution of AM calcein.
The amplified product (223 bp) was electrophoresed in 2% agarose gel stained with ethidium
The mutant (EtBr-02) obtained after ethidium
bromide treatment gave 2.
harzianum JF419706 by ethidium
bromide and UV radiation.
A more detailed methodology has been described that uses calcein-AM to monitor extracellular enzyme activity and ethidium
homodimer-1 to measure cell membrane integrity.
aureus confers resistance to a broad spectrum of compounds including fluoroquinolones, reserpine, and ethidium
bromide (Poole 2000; DeMarco et al.
8% agarose gels, stained with ethidium
bromide and observed under UV light.
x 3H2O, RPMI, 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide, yellow tetrazole (MTT), penicillin-streptomycin, DAPI (4_,6-diamidino-2-phenylindole, dihydrochloride), ethidium
bromide and Acridine orange were all purchased from Sigma-Aldrich.
A small amount of resulting PCR product (2 [micro]L) was separated on 4% agarose gels and visualized by ethidium
bromide staining to confirm success of PCR.