ethidium bromide

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ethidium bromide

[e′thid·ē·əm ′brō‚mīd]
(organic chemistry)
C21H20BrN3 Dark red crystals with a melting point of 238-240°C; used in treating trypanosomiasis in animals and as an inhibitor of deoxyribonucleic and ribonucleic acid synthesis. Also known as homidium bromide.
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as there is no loss in sensitivity compared to UV and ethidium bromide
A combination assay between the 13 compounds and the antibiotics erythromycin, tetracycline and oxacillin, and ethidium bromide (EtBr) was performed to search for antibiotic modulatory activities.
The rosamultic acid-treated cells were also stained with acridine orange and ethidium bromide and visualized under the fluorescence microscope.
PCR products were separated in 3% agarose gels run in standard TAE buffer and visualized by ethidium bromide staining.
2015 Deep Market Research Report on Global Ethidium Bromid Industry : For international and China market analysis, the report analyzes Ethidium Bromid markets in China and other countries or regions (such as US, Europe, Japan, etc) by presenting research on global products of different types and applications developments and trends of market, technology, and competitive landscape, and leading suppliers and countries'2009-2014 capacity, production, cost, price, profit, production value, and gross margin.
7% agarose gel containing ethidium bromide and/or nonmutagenic fluorescent DNA dye (EZ Vision In-Gel Solution.
The products were fractionated on 2% agarose gel and stained with ethidium bromide.
The presence of a 259 bp amplicon covering codons 39 to 123 of gyrA was confirmed by 1% agarose gel electrophoresis, ethidium bromide staining and UV transillumination.
Ethidium bromide is weakly fluorescent when free in water or buffer, but can intercalate (slide between) DNA base pairs in dsDNA; upon doing so, and being isolated from the aqueous environment, it has a large increase in quantum yield.
When Robert Watson, who was working with Russell Higuchi, accidently ran a PCR in the presence of ethidium bromide, which was intended for post-PCR gel staining, and found that the reaction was not totally inhibited, they realized it should be possible to monitor the amplification in real time via the dye's fluorescence (3).
After electrophoresis, the gel was soaked into ethidium bromide staining solution (40 [micro]l of ethidium bromide in 1 liter of water or TBE) for 30 minutes.
5M EDTA [pH 7]), distilled water to 1000 mL), followed by ethidium bromide staining (0.