immunoassay

(redirected from FPIA)
Also found in: Dictionary, Thesaurus, Medical, Acronyms.

Immunoassay

An assay that quantifies antigen or antibody by immunochemical means. The antigen can be a relatively simple substance such as a drug, or a complex one such as a protein or a virus. See Antibody, Antigen

The reactants are first mixed so that a varying quantity of one (A) is added to a constant amount of the other (B). The formation of an immune (antigen-antibody) complex is measured as a function of the varied reactant (A). The result is represented by a “standard curve” for reactant A. An unknown sample is tested by adding it to reactant B. The extent of the measured change is referred to the standard curve, and thereby is obtained the amount of reactant A which produces a comparable change. The amount is represented as the content of reactant A in the unknown sample. See Immunofluorescence, Immunology, Radioimmunoassay

immunoassay

[‚im·yə·nō′a‚sāy]
(immunology)
A laboratory detection method that uses antibodies to react with specific substances.
References in periodicals archive ?
[3] Nonstandard abbreviations: HDMTX, high-dose methotrexate; MTX, methotrexate; [CPDG.sub.2], carboxypeptidase G2; DAMPA, 2,4-diamino-[N.sup.10]-methylpteroic acid; FPIA, fluorescence polarization immunoassay; LC-MS/MS, liquid chromatography-tandem mass spectrometry.
In surveys 2 and 3, tHcy was measured by the FPIA method 7 of 23 and 6 of 17 times, respectively.
Response of AxSYM FPIA assay for amphetamines after addition of heptaminol to drug-free urine.
We can also exclude that the higher MEGX concentrations observed in uremic patients were the result of positive interferences with the TDx FPIA by substances that had accumulated in uremic plasma because the predose (0 min) value was subtracted from values obtained at the various postinjection times.
Abbreviations: EPIC: the European Prospective Investigation into Cancer and Nutrition; vs: versus; M: male; F: female; PB: population-based; HB: hospital-based; HPLC: High Performance Liquid Chromatography; GC-MS: gas chromatography-mass spectrometry; FPIA: fluorescence polarization immunoassay.
The analysis of cortisol was performed on an Architect i2000[TM] immunochemistry analyzer (Abbott, Chicago, IL, USA) using the fluorescence polarization immunoassay FPIA (Abbott).
Serum hormone concentration for qualitative determination of GH was done by Enzyme Amplified Sensitivity Immunoassay, T3 was done by using Micro Particle Enzyme Immuno Assay (MEIA), while T4 was analyzed using Fluorescence Polarization Immunoassay (FPIA).
The plasma total homocysteine levels were measured with fluorescence polarization immunoassay, FPIA in the IMX System.
Total homocysteine concentration in plasma was determined by an immunochemical method with the fluorescence polarization immunoassay (FPIA) using an IMx analyzer and special ABBOTT sets.